SDS-PAGE (SDS), ELISA, Western Blotting (WB), Mass Spectrometry (MS)
原理
Recombinant Human NT5E Protein is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
NT5E
宿主: 大鼠
宿主: 人细胞
Recombinant
> 90 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, 10 % Glycerol, pH 8.0.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
-20 °C
储存方法
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.