CD73 Protein (AA 27-554) (His tag)
-
- 抗原 See all CD73 (NT5E) 蛋白
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-554
-
宿主
- Cynomolgus
-
资源
- HEK-293 Cells
- 标记
- This CD73 protein is labelled with His tag.
- 原理
- Cynomolgus CD73/NT5E Protein
- 序列
- Trp27-Cys554
- 产品特性
- Recombinant Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Cys554.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product NT5E 蛋白
-
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) protein (His tag)
NT5E 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (His tag)NT5E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (Fc Tag)NT5E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (His tag)NT5E 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 34-214) protein (His tag)NT5E 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
5'-Nucleotidase, Ecto (CD73) (NT5E) protein (His tag)NT5E 宿主: 大鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 120 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- 别名
- CD73 (NT5E 产品)
- 别名
- CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
- 背景
- CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
- 分子量
- 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- 途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
-
