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CD73 Protein (AA 27-549) (His tag,AVI tag,Biotin)

NT5E 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
产品编号 ABIN6810044
发货至: 中国
  • 抗原 See all CD73 (NT5E) 蛋白
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 27-549
    宿主
    • 15
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    资源
    • 16
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    标记
    This CD73 protein is labelled with His tag,AVI tag,Biotin.
    序列
    AA 27-549
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    纯度
    >90 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product NT5E 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 120 mM NaCl, pH 7.5
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    别名
    CD73 (NT5E 产品)
    别名
    CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
    背景
    5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
    分子量
    61.7 kDa
    途径
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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