FZD4 Protein (AA 37-537) (rho-1D4 tag)
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- 抗原 See all FZD4 蛋白
- FZD4 (Frizzled Family Receptor 4 (FZD4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 37-537
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This FZD4 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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FGDEEERRCD PIRIAMCQNL GYNVTKMPNL VGHELQTDAE LQLTTFTPLI QYGCSSQLQF FLCSVYVPMC TEKINIPIGP CGGMCLSVKR RCEPVLREFG FAWPDTLNCS KFPPQNDHNH MCMEGPGDEE VPLPHKTPIQ PGEECHSVGS NSDQYIWVKR SLNCVLKCGY DAGLYSRSAK EFTDIWMAVW ASLCFISTTF TVLTFLIDSS RFSYPERPII FLSMCYNIYS IAYIVRLTVG RERISCDFEE AAEPVLIQEG LKNTGCAIIF LLMYFFGMAS SIWWVILTLT WFLAAGLKWG HEAIEMHSSY FHIAAWAIPA VKTIVILIMR LVDADELTGL CYVGNQNLDA LTGFVVAPLF TYLVIGTLFI AAGLVALFKI RSNLQKDGTK TDKLERLMVK IGVFSVLYTV PATCVIACYF YEISNWALFR YSADDSNMAV EMLKIFMSLL VGITSGMWIW SAKTLHTWQK CSNRLVNSGK VKREKRGNGW VKPGKGNETV V
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Fzd4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product FZD4 蛋白
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Frizzled Family Receptor 4 (FZD4) protein (His tag)
FZD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Frizzled Family Receptor 4 (FZD4) (AA 107-206) protein (GST tag)FZD4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Frizzled Family Receptor 4 (FZD4) (AA 1-537) protein (GST tag)FZD4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Frizzled Family Receptor 4 (FZD4) (AA 37-222) protein (His tag)Crystallography grade FZD4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Frizzled Family Receptor 4 (FZD4) (AA 37-222) protein (His tag)Crystallography grade FZD4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Frizzled Family Receptor 4 (FZD4) (AA 37-537) protein (rho-1D4 tag)Crystallography grade FZD4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- FZD4 (Frizzled Family Receptor 4 (FZD4))
- 别名
- Fzd4 (FZD4 产品)
- 别名
- CG4626 Protein, DFz4 Protein, Dfz4 Protein, Dm Fz4 Protein, Dmel\\CG4626 Protein, Fz4 Protein, anon-WO0170980.10 Protein, anon-WO0170980.11 Protein, fz1 Protein, zg01 Protein, CD344 Protein, EVR1 Protein, FEVR Protein, FZD4S Protein, Fz-4 Protein, FzE4 Protein, GPCR Protein, hFz4 Protein, frizzled4 Protein, fz4 Protein, FZ-4 Protein, frizzled 4 Protein, frizzled class receptor 4 Protein, frizzled-4 Protein, frizzled class receptor 4 S homeolog Protein, fz4 Protein, fzd4 Protein, Tsp_10376 Protein, FZD4 Protein, Fzd4 Protein, fzd4.S Protein
- 背景
- Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. {ECO:0000269|PubMed:10097073, ECO:0000269|PubMed:19837033}.
- 分子量
- 57.7 kDa Including tag.
- UniProt
- Q61088
- 途径
- WNT signaling, Hormone Transport, Sensory Perception of Sound
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