EPH Receptor B6 Protein (EPHB6) (AA 33-1014) (rho-1D4 tag)
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- 抗原 See all EPH Receptor B6 (EPHB6) 蛋白
- EPH Receptor B6 (EPHB6)
- 蛋白类型
- Recombinant
- 产品特性
- AA 33-1014
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This EPH Receptor B6 protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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LEEVLLDTTG ETSEIGWLTY PPGGWDEVSV LDDQRRLTRT FEACHVAGLP PGSGQDNWLQ THFVERRGAQ RAHIRLHFSV RACSSLGVSG GTCRETFTLY YRQADEPDGP DSIAAWHLKR WTKVDTIAAD ESFPASSSSS SWAVGPHRTG QRVGLQLNVK ERSFGPLTQR GFYVAFQDTG ACLALVAVKL FSYTCPSVLR AFASFPETQA SGAGGASLVA AVGTCVAHAE PEEDGVGGQA GGSPPRLHCN GEGRWMVAVG GCRCQPGHQP ARGDKLCQAC PEGSYKALAG NVPCSPCPAR SHSPDPAAPV CPCLQGFYRA SSDPPEAPCT GPPSAPRELW FEVQGSALML HWRLPQELGG RGDLLFNVVC KECGGHGEPS SGGMCRRCRD EVHFDPRQRG LTESRVLVGG LRAHVPYILE VQAVNGVSEL SPDPPQAAAI NVSTSHEVPS AVPVMHQVSR AANSITVSWP QPEQTNGNIL DYQLRYYDQA EDESHSFTMT SETNTATVTR LSPGHIYGFQ VRARTAAGHG PYGGKVYFQT LPQGELSSQL PEKLSLVIGS ILGALAFLLL AAITVLAVIF QRKRRGTGYT EQLQQYSSPG LGVKYYIDPS TYDDPCQAIR ELAREVDPTY IKIEEVIGAG SFGEVRRGRL QPRGRREQAV AIQALWAGGA ESLKMTFLGR AALLGQFQHP NILRLEGVVT KSRPVMVLTE LMELGPLDSF LRQREGQFSS LQLVAMQRGV AAAMQYLSSF AFVHRALSAR SVLVNSHLVC KVARLGHSPQ GSSSLLRWAA PEVITHGKYT TSSDVWSFGI LMWEVMSYGE RPYWDMNEQE VLNAIEQEFR LPPPPGCPPG LHLLMLDTWQ KDRARRPHFD QLVAAFDKMI RKPDTLQAEG GSGDRPSQAL LNPVALDFPC LDSPQAWLSA IGLECYQDNF SKFGLSTFSD VAQLSLEDLP GLGITLAGHQ KKLLHNIQLL QQHLRQPGSV EV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ephb6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EPHB6 蛋白
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EPH Receptor B6 (EPHB6) (AA 23-122) protein (GST tag)
EPHB6 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
EPH Receptor B6 (EPHB6) (AA 616-1021) protein (His tag)Crystallography grade EPHB6 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
EPH Receptor B6 (EPHB6) (AA 613-1014) protein (His tag)Crystallography grade EPHB6 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
EPH Receptor B6 (EPHB6) (AA 32-594) protein (His tag)Crystallography grade EPHB6 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
EPH Receptor B6 (EPHB6) (AA 33-591) protein (His tag)Crystallography grade EPHB6 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
EPH Receptor B6 (EPHB6) (AA 32-1021) protein (rho-1D4 tag)Crystallography grade EPHB6 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
EPH Receptor B6 (EPHB6) protein (DYKDDDDK-His Tag)EPHB6 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EPH Receptor B6 (EPHB6)
- 别名
- Ephb6 (EPHB6 产品)
- 别名
- CEK9 Protein, EPHB5 Protein, Cekl Protein, Mep Protein, HEP Protein, EPH receptor B6 Protein, Eph receptor B6 Protein, EPHB6 Protein, Ephb6 Protein
- 背景
- Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2 (By similarity). {ECO:0000250}.
- 分子量
- 108.1 kDa Including tag.
- UniProt
- O08644
- 途径
- RTK signaling, Hormone Transport
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