Ret Proto-Oncogene Protein (RET) (AA 660-1115) (His tag)
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- 抗原 See all Ret Proto-Oncogene (RET) 蛋白
- Ret Proto-Oncogene (RET)
- 蛋白类型
- Recombinant
- 产品特性
- AA 660-1115
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This Ret Proto-Oncogene protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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HHHKHGHKPP IASAEMTFCR PAQGFPISYS SSGTRRPSLD STENQVPVDS FKIPEDPKWE FPRKNLVLGK TLGEGEFGKV VKATAFRLKG RAGYTTVAVK MLKENASQSE LRDLLSEFNL LKQVNHPHVI KLYGACSQDG PLLLIVEYAK YGSLRGFLRD SRKIGPAYVS GGGSRNSSSL DHPDERVLTM GDLISFAWQI SRGMQYLAEM KLVHRDLAAR NILVAEGRKM KISDFGLSRD VYEEDSYVKK SKGRIPVKWM AIESLFDHIY TTQSDVWSFG VLLWEIVTLG GNPYPGIPPE RLFNLLKTGH RMERPDNCSE EMYRLMLQCW KQEPDKRPVF ADISKDLEKM MVKSRDYLDL AASTPSDSLL YDDGLSEEET PLVDCNNAPL PRSLPSTWIE NKLYGMSDPN WPGESPVPLT RADGTSTGFP RYANDSVYAN WMVSPSAAKL MDTFDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ret Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Ret Proto-Oncogene (RET)
- 别名
- Ret (RET 产品)
- 别名
- c-ret Protein, cret Protein, etID315074.13 Protein, ret1 Protein, wu:fd13h01 Protein, X-ret Protein, ret-A Protein, xret Protein, RET Protein, MTC1 Protein, CDHF12 Protein, CDHR16 Protein, HSCR1 Protein, MEN2A Protein, MEN2B Protein, PTC Protein, RET-ELE1 Protein, RET51 Protein, RET9 Protein, c-Ret Protein, ret proto-oncogene receptor tyrosine kinase Protein, ret proto-oncogene S homeolog Protein, ret proto-oncogene Protein, ret Protein, ret.S Protein, RET Protein, Ret Protein
- 背景
- Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers, these diseases are characterized by aberrant integrins-regulated cell migration. {ECO:0000269|PubMed:17322904, ECO:0000269|PubMed:20237269}.
- 分子量
- 52.2 kDa Including tag.
- UniProt
- P35546
- 途径
- RTK signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
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