Thimet Oligopeptidase 1 Protein (THOP1) (AA 1-687) (Strep Tag)
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- 抗原 See all Thimet Oligopeptidase 1 (THOP1) 蛋白
- Thimet Oligopeptidase 1 (THOP1)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-687
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This Thimet Oligopeptidase 1 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MKPPAACAGD VVDAASPAST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQNFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP CKDIRAASTE ADKKLSEFDV EMSMRQDVYQ RVVWLQEKTP KNSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP EDFLSSLEKA EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE ENCAILKELV SLRAQKSSLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAVAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGSE APQDLLEKLI KSRQANAGLF NLRQIVLAKV DQVLHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASAMLKQ FLGRDPKQDA FLLSKGLQVE GSEAPAC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product THOP1 蛋白
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Thimet Oligopeptidase 1 (THOP1) (AA 1-689) protein (GST tag)
THOP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Thimet Oligopeptidase 1 (THOP1) (AA 255-354) protein (GST tag)THOP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Thimet Oligopeptidase 1 (THOP1) (Active) protein (His tag)THOP1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
Thimet Oligopeptidase 1 (THOP1) (AA 1-689) protein (Strep Tag)Crystallography grade THOP1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Thimet Oligopeptidase 1 (THOP1) protein (Myc-DYKDDDDK Tag)THOP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Thimet Oligopeptidase 1 (THOP1)
- 别名
- Thop1 (THOP1 产品)
- 别名
- EP24.15 Protein, MEPD_HUMAN Protein, MP78 Protein, TOP Protein, AI131655 Protein, AI327041 Protein, THOP1 Protein, thop1 Protein, MGC147297 Protein, LOC397806 Protein, mepd Protein, thimet oligopeptidase 1 Protein, thimet oligopeptidase 1 L homeolog Protein, thimet oligopeptidase Protein, peptidase Protein, Thimet oligopeptidase Protein, THOP1 Protein, Thop1 Protein, thop1 Protein, thop1.L Protein, Tc00.1047053506411.10 Protein, Tc00.1047053511237.10 Protein, Noca_4613 Protein, ANI_1_1400064 Protein, MBOO_RS10705 Protein, AOR_1_880174 Protein, AnaeK_0425 Protein, MPAL_RS03920 Protein, Hoch_5061 Protein, mepd Protein
- 背景
- Thimet oligopeptidase (EC 3.4.24.15),FUNCTION: Involved in the metabolism of neuropeptides under 20 amino acid residues long (PubMed:10969067). Involved in cytoplasmic peptide degradation. Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments (By similarity). Also acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity). {ECO:0000250|UniProtKB:P24155, ECO:0000250|UniProtKB:P52888, ECO:0000269|PubMed:10969067}.
- 分子量
- 78.0 kDa
- UniProt
- Q8C1A5
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