Thimet Oligopeptidase 1 Protein (THOP1) (His tag)
-
- 抗原 See all Thimet Oligopeptidase 1 (THOP1) 蛋白
- Thimet Oligopeptidase 1 (THOP1)
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 小鼠
-
资源
- Baculovirus infected Insect Cells
- 标记
- This Thimet Oligopeptidase 1 protein is labelled with His tag.
- 原理
- Recombinant Mouse THOP1 Protein (His Tag)(Active)
- 序列
- Lys 2-Cys 687
- 产品特性
- A DNA sequence encoding the mouse THOP1 (NP_073144.3) (Lys 2-Cys 687) was fused with a polyhistidine tag at the N-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH or Mca-PLGPK(Dnp)-OH.The specific activity is > 100 pmoles/min/μg.
- Top Product
- Discover our top product THOP1 蛋白
-
Thimet Oligopeptidase 1 (THOP1) (AA 1-689) protein (GST tag)
THOP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Thimet Oligopeptidase 1 (THOP1) (AA 255-354) protein (GST tag)THOP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Thimet Oligopeptidase 1 (THOP1) (AA 1-689) protein (Strep Tag)Crystallography grade THOP1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Thimet Oligopeptidase 1 (THOP1) (AA 1-687) protein (Strep Tag)Crystallography grade THOP1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Thimet Oligopeptidase 1 (THOP1) protein (Myc-DYKDDDDK Tag)THOP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Thimet Oligopeptidase 1 (THOP1)
- 别名
- THOP1 (THOP1 产品)
- 别名
- EP24.15 Protein, MEPD_HUMAN Protein, MP78 Protein, TOP Protein, AI131655 Protein, AI327041 Protein, THOP1 Protein, thop1 Protein, MGC147297 Protein, LOC397806 Protein, mepd Protein, thimet oligopeptidase 1 Protein, thimet oligopeptidase 1 L homeolog Protein, thimet oligopeptidase Protein, peptidase Protein, Thimet oligopeptidase Protein, THOP1 Protein, Thop1 Protein, thop1 Protein, thop1.L Protein, Tc00.1047053506411.10 Protein, Tc00.1047053511237.10 Protein, Noca_4613 Protein, ANI_1_1400064 Protein, MBOO_RS10705 Protein, AOR_1_880174 Protein, AnaeK_0425 Protein, MPAL_RS03920 Protein, Hoch_5061 Protein, mepd Protein
- 背景
-
Background: THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 3.4.24.15, or EP24.15, is a zinc(II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.
Synonym: AI131655,AI327041,EP24.15
- 分子量
- 80.1 kDa
- NCBI登录号
- NP_073144
-
