EPH Receptor A4 Protein (EPHA4) (AA 20-986) (rho-1D4 tag)
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- 抗原 See all EPH Receptor A4 (EPHA4) 蛋白
- EPH Receptor A4 (EPHA4)
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-986
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宿主
- 人
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资源
- Insect Cells
- 标记
- This EPH Receptor A4 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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VTGSRVYPAN EVTLLDSRSV QGELGWIASP LEGGWEEVSI MDEKNTPIRT YQVCNVMEPS QNNWLRTDWI TREGAQRVYI EIKFTLRDCN SLPGVMGTCK ETFNLYYYES DNDKERFIRE NQFVKIDTIA ADESFTQVDI GDRIMKLNTE IRDVGPLSKK GFYLAFQDVG ACIALVSVRV FYKKCPLTVR NLAQFPDTIT GADTSSLVEV RGSCVNNSEE KDVPKMYCGA DGEWLVPIGN CLCNAGHEER SGECQACKIG YYKALSTDAT CAKCPPHSYS VWEGATSCTC DRGFFRADND AASMPCTRPP SAPLNLISNV NETSVNLEWS SPQNTGGRQD ISYNVVCKKC GAGDPSKCRP CGSGVHYTPQ QNGLKTTKVS ITDLLAHTNY TFEIWAVNGV SKYNPNPDQS VSVTVTTNQA APSSIALVQA KEVTRYSVAL AWLEPDRPNG VILEYEVKYY EKDQNERSYR IVRTAARNTD IKGLNPLTSY VFHVRARTAA GYGDFSEPLE VTTNTVPSRI IGDGANSTVL LVSVSGSVVL VVILIAAFVI SRRRSKYSKA KQEADEEKHL NQGVRTYVDP FTYEDPNQAV REFAKEIDAS CIKIEKVIGV GEFGEVCSGR LKVPGKREIC VAIKTLKAGY TDKQRRDFLS EASIMGQFDH PNIIHLEGVV TKCKPVMIIT EYMENGSLDA FLRKNDGRFT VIQLVGMLRG IGSGMKYLSD MSYVHRDLAA RNILVNSNLV CKVSDFGMSR VLEDDPEAAY TTRGGKIPIR WTAPEAIAYR KFTSASDVWS YGIVMWEVMS YGERPYWDMS NQDVIKAIEE GYRLPPPMDC PIALHQLMLD CWQKERSDRP KFGQIVNMLD KLIRNPNSLK RTGTESSRPN TALLDPSSPE FSAVVSVGDW LQAIKMDRYK DNFTAAGYTT LEAVVHVNQE DLARIGITAI THQNKILSSV QAMRTQMQQM HGRMVPV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human EPHA4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EPHA4 蛋白
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EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)
EPHA4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor A4 (EPHA4) (AA 20-547) protein (His tag)EPHA4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor A4 (EPHA4) (AA 586-986) (Active) protein (GST tag)EPHA4 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
EPH Receptor A4 (EPHA4) (AA 570-986) (Active) protein (His-GST)EPHA4 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
EPH Receptor A4 (EPHA4) (AA 887-986) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 1-105) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 1-986) protein (GST tag)EPHA4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor A4 (EPHA4) (AA 570-986) (Active) protein (GST tag,His tag)EPHA4 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 99 % as determined by reducing SDS-PAGE. Active
EPH Receptor A4 (EPHA4) (Active) protein (His tag)EPHA4 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
EPH Receptor A4 (EPHA4) (Active) protein (Fc Tag)EPHA4 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EPH Receptor A4 (EPHA4)
- 别名
- EPHA4 (EPHA4 产品)
- 别名
- HEK8 Protein, SEK Protein, TYRO1 Protein, RGD1560587 Protein, ephe Protein, EPHA4 Protein, Ci-Eph1 Protein, sek Protein, hek8 Protein, rtk1 Protein, tyr01 Protein, 2900005C20Rik Protein, AI385584 Protein, Cek8 Protein, Hek8 Protein, Sek Protein, Sek1 Protein, Tyro1 Protein, rb Protein, XEpha4 Protein, sek-1 Protein, epha4 Protein, pag Protein, EphA4 Protein, apha3 Protein, ek2 Protein, epha3 Protein, sb:cb246 Protein, zek2 Protein, zgc:109882 Protein, EPH receptor A4 Protein, Eph receptor A4 Protein, ephrin receptor epsilon Protein, EPH receptor A4 S homeolog Protein, EPH receptor A4 L homeolog Protein, eph receptor A4a Protein, EPHA4 Protein, Epha4 Protein, ephe Protein, epha4 Protein, epha4.S Protein, epha4.L Protein, epha4a Protein
- 背景
- Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. {ECO:0000269|PubMed:17143272}.
- 分子量
- 109.0 kDa Including tag.
- UniProt
- P54764
- 途径
- RTK signaling
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