Transferrin Receptor Protein (AA 101-760) (rho-1D4 tag)
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- 抗原 See all Transferrin Receptor (TFRC) 蛋白
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 蛋白类型
- Recombinant
- 产品特性
- AA 101-760
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Transferrin Receptor protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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LAGTESPVRE EPGEDFPAAR RLYWDDLKRK LSEKLDSTDF TGTIKLLNEN SYVPREAGSQ KDENLALYVE NQFREFKLSK VWRDQHFVKI QVKDSAQNSV IIVDKNGRLV YLVENPGGYV AYSKAATVTG KLVHANFGTK KDFEDLYTPV NGSIVIVRAG KITFAEKVAN AESLNAIGVL IYMDQTKFPI VNAELSFFGH AHLGTGDPYT PGFPSFNHTQ FPPSRSSGLP NIPVQTISRA AAEKLFGNME GDCPSDWKTD STCRMVTSES KNVKLTVSNV LKEIKILNIF GVIKGFVEPD HYVVVGAQRD AWGPGAAKSG VGTALLLKLA QMFSDMVLKD GFQPSRSIIF ASWSAGDFGS VGATEWLEGY LSSLHLKAFT YINLDKAVLG TSNFKVSASP LLYTLIEKTM QNVKHPVTGQ FLYQDSNWAS KVEKLTLDNA AFPFLAYSGI PAVSFCFCED TDYPYLGTTM DTYKELIERI PELNKVARAA AEVAGQFVIK LTHDVELNLD YERYNSQLLS FVRDLNQYRA DIKEMGLSLQ WLYSARGDFF RATSRLTTDF GNAEKTDRFV MKKLNDRVMR VEYHFLSPYV SPKESPFRHV FWGSGSHTLP ALLENLKLRK QNNGAFNETL FRNQLALATW TIQGAANALS GDVWDIDNEF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TFRC Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product TFRC 蛋白
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Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (Fc Tag)
TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transferrin Receptor (p90, CD71) (TFRC) protein (His-Avi Tag)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-Avi Tag,Biotin)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-763) protein (His tag)Crystallography grade TFRC 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His tag)TFRC 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-DYKDDDDK-Strep II Tag)TFRC 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-760) protein (Strep Tag)Crystallography grade TFRC 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-763) protein (Strep Tag)Crystallography grade TFRC 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- TFRC (TFRC 产品)
- 别名
- Trfr Protein, LOC100219280 Protein, tfr Protein, cd71 Protein, tfr1 Protein, trfr Protein, CD71 Protein, T9 Protein, TFR Protein, TFR1 Protein, TR Protein, TRFR Protein, p90 Protein, 2610028K12Rik Protein, AI195355 Protein, AI426448 Protein, AU015758 Protein, E430033M20Rik Protein, Mtvr-1 Protein, Mtvr1 Protein, transferrin receptor Protein, TFRC Protein, Tfrc Protein, tfrc Protein
- 背景
- Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. {ECO:0000250, ECO:0000269|PubMed:3568132}., (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. {ECO:0000269|PubMed:17287727, ECO:0000269|PubMed:18268337}.
- 分子量
- 75.0 kDa Including tag.
- UniProt
- P02786
- 途径
- Transition Metal Ion Homeostasis
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