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Transferrin Receptor Protein (AA 89-760) (Fc Tag)

TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
产品编号 ABIN7275701
发货至: 中国
  • 抗原 See all Transferrin Receptor (TFRC) 蛋白
    Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
    蛋白类型
    Recombinant
    产品特性
    AA 89-760
    宿主
    • 17
    • 6
    • 4
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    • 1
    资源
    • 16
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    HEK-293 Cells
    标记
    This Transferrin Receptor protein is labelled with Fc Tag.
    原理
    Human Transferrin R/CD71 Protein
    序列
    Cys89-Phe760
    产品特性
    Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys89-Phe760.
    纯度
    > 95 % as determined by Tris-Bis PAGE
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human Transferrin R, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. The affinity constant of 0.1μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
    别名
    Transferrin R (TFRC 产品)
    别名
    Trfr Protein, LOC100219280 Protein, tfr Protein, cd71 Protein, tfr1 Protein, trfr Protein, CD71 Protein, T9 Protein, TFR Protein, TFR1 Protein, TR Protein, TRFR Protein, p90 Protein, 2610028K12Rik Protein, AI195355 Protein, AI426448 Protein, AU015758 Protein, E430033M20Rik Protein, Mtvr-1 Protein, Mtvr1 Protein, transferrin receptor Protein, TFRC Protein, Tfrc Protein, tfrc Protein
    背景
    The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
    分子量
    101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Tris-Bis PAGE result.
    途径
    Transition Metal Ion Homeostasis
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