Transferrin Receptor Protein (AA 89-760) (hFc Tag)
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Quick Overview for Transferrin Receptor Protein (AA 89-760) (hFc Tag) (ABIN7275701)
抗原
See all Transferrin Receptor (TFRC) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 89-760
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标记
- This Transferrin Receptor protein is labelled with hFc Tag.
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原理
- Human Transferrin R/CD71 Protein
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序列
- Cys89-Phe760
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产品特性
- Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys89-Phe760.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human Transferrin, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Transferrin R, hFc Tag with the EC50 of 58.4ng/ml determined by ELISA.,Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.,Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-Avi Tag)
TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) (Active) protein (Biotin,His-Avi Tag)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE Active
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-763) protein (His tag)custom-made TFRC 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-760) protein (His tag)custom-made TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-763) protein (His tag)TFRC 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His tag)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-DYKDDDDK-Strep II Tag)TFRC 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) (Active) protein (His tag)TFRC 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- Transferrin R
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背景
- The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
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分子量
- 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Bis-Tris PAGE result.
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UniProt
- P02786
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途径
- Transition Metal Ion Homeostasis
抗原
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