Transferrin Receptor Protein (AA 89-760) (His-Avi Tag)
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Quick Overview for Transferrin Receptor Protein (AA 89-760) (His-Avi Tag) (ABIN7275702)
抗原
See all Transferrin Receptor (TFRC) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 89-760
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标记
- This Transferrin Receptor protein is labelled with His-Avi Tag.
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原理
- Human Transferrin R/CD71 Protein
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序列
- Cys89-Phe760
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产品特性
- Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human Transferrin R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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!探索我们的预定义定制蛋白和定制蛋白服务!Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 150,642.20 ¥您的项目需要进一步定制吗?联系我们,了解我们的定制蛋白解决方案
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- Transferrin R
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背景
- The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
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分子量
- 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Tris-Bis PAGE result.
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UniProt
- P02786
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途径
- Transition Metal Ion Homeostasis
抗原
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