Transferrin Receptor Protein (AA 89-760) (Biotin,His-Avi Tag)
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Quick Overview for Transferrin Receptor Protein (AA 89-760) (Biotin,His-Avi Tag) (ABIN7275703)
抗原
See all Transferrin Receptor (TFRC) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 89-760
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标记
- This Transferrin Receptor protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human Transferrin R/CD71 Protein
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序列
- Cys89-Phe760
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产品特性
- Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).,Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).,Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.,Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.,Loaded Anti-Transferrin R Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator® Prime).
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 150,642.20 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) (Active) protein (hFc Tag)
TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-Avi Tag)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-763) protein (His tag)custom-made TFRC 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transferrin Receptor (p90, CD71) (TFRC) (AA 1-760) protein (His tag)custom-made TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-763) protein (His tag)TFRC 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (GST tag)TFRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His tag)TFRC 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) protein (His-DYKDDDDK-Strep II Tag)TFRC 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Transferrin Receptor (p90, CD71) (TFRC) (AA 89-760) (Active) protein (His tag)TFRC 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine, 8 % trehalose ( pH 7.4).
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- Transferrin R
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背景
- The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
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分子量
- 77.9 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
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UniProt
- P02786
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途径
- Transition Metal Ion Homeostasis
抗原
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