AXL Protein (AA 473-894) (His tag)
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- 抗原 See all AXL 蛋白
- AXL (AXL Receptor tyrosine Kinase (AXL))
- 蛋白类型
- Recombinant
- 产品特性
- AA 473-894
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宿主
- 人
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资源
- Insect Cells
- 标记
- This AXL protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
- MHHHHHHHRR KKETRYGEVF EPTVERGELV VRYRVRKSYS RRTTEATLNSL GISEELKEKL RDVMVDRHKV ALGKTLGEGE FGAVMEGQLN QDDSILKVAV KTMKIAICTR SELEDFLSEA VCMKEFDHPN VMRLIGVCFQ GSERESFPAP VVILPFMKHG DLHSFLLYSR LGDQPVYLPT QMLVKFMADI ASGMEYLSTK RFIHRDLAAR NCMLNENMSV CVADFGLSKK IYNGDYYRQG RIAKMPVKWI AIESLADRVY TSKSDVWSFG VTMWEIATRG QTPYPGVENS EIYDYLRQGN RLKQPADCLD GLYALMSRCW ELNPQDRPSF TELREDLENT LKALPPAQEP DEILYVNMDE GGGYPEPPGA AGGADPPTQP DPKDSCSCLT AAEVHPAGRY VLCPSTTPSP AQPADRGSPA APGQEDGA
- 产品特性
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The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product AXL 蛋白
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AXL Receptor tyrosine Kinase (AXL) (AA 33-442) protein (His tag)
AXL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) (AA 33-449) protein (His tag)AXL 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) protein (His-Avi Tag)AXL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
AXL Receptor tyrosine Kinase (AXL) (AA 26-449) protein (Fc Tag)AXL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) protein (His-Avi Tag,Biotin)AXL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) (AA 26-449) protein (Fc Tag,Biotin)AXL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) (AA 20-445) protein (His tag)AXL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
AXL Receptor tyrosine Kinase (AXL) (AA 464-885) (Active) protein (GST tag)AXL 宿主: 人 宿主: Insect Cells Recombinant SDS, Func Active
AXL Receptor tyrosine Kinase (AXL) (AA 464-885) (Active) protein (His-GST)AXL 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
AXL Receptor tyrosine Kinase (AXL) (AA 1-894) protein (GST tag)AXL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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N-terminal His-tag
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 20 mM Hepes, pH 7.5; 50 mM NaCl; 10 % Glycerol
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- AXL (AXL Receptor tyrosine Kinase (AXL))
- 别名
- AXL (AXL 产品)
- 别名
- JTK11 Protein, UFO Protein, AI323647 Protein, Ark Protein, Tyro7 Protein, Ufo Protein, AXL receptor tyrosine kinase Protein, Axl receptor tyrosine kinase Protein, AXL Protein, Axl Protein
- 背景
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3, but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. {ECO:0000269|PubMed:10403904, ECO:0000269|PubMed:11484958, ECO:0000269|PubMed:12364394, ECO:0000269|PubMed:12490074, ECO:0000269|PubMed:15507525, ECO:0000269|PubMed:15733062, ECO:0000269|PubMed:1656220, ECO:0000269|PubMed:18840707}., (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope (PubMed:22156524, PubMed:22673088, PubMed:25277499, PubMed:21501828). Acts as a receptor for ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope (PubMed:17005688). {ECO:0000269|PubMed:17005688, ECO:0000269|PubMed:21501828, ECO:0000269|PubMed:22156524, ECO:0000269|PubMed:22673088, ECO:0000269|PubMed:25277499}.
- 分子量
- 48.2 kDa Including tag.
- UniProt
- P30530
- 途径
- RTK signaling, Cellular Response to Molecule of Bacterial Origin
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