MUSK Protein (AA 24-495) (His tag)
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- 抗原 See all MUSK 蛋白
- MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-495
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宿主
- 人
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资源
- Insect Cells
- 标记
- This MUSK protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
- MLPKAPVITT PLETVDALVE EVATFMCAVE SYPQPEISWT RNKILIKLF DTRYSIRENG QLLTILSVE DSDDGIYCCT ANNGVGGAVE SCGALQVKMK PKITRPPINV KIIEGLKAVL PCTTMGNPKP SVSWIKGDSP LRENSRIAVL ESGSLRIHNV QKEDAGQYRC VAKNSLGTAY SKVVKLEVEV FARILRAPES HNVTFGSFVT LHCTATGIPV PTITWIENGN AVSSGSIQES VKDRVIDSRL QLFITKPGLY TCIATNKHGE KFSTAKAAAT ISIAEWSKPQ KDNKGYCAQY RGEVCNAVLA KDALVFLNTS YADPEEAQEL LVHTAWNELKV VSPVCRPAAE ALLCNHIFQE CSPGVVPTPIP ICREYCLAVKE LFCAKEWLVM EEKTHRGLYR SEMHLLSVPE CSKLPSMHWD PTACARLPHL DYNKENLKTF PPMTSSKPSVD IPNLPSSSSS SFSVSPTYSM THHHHHH
- 产品特性
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The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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C-terminal His-tag
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 20 mM Hepes, pH7.4; 100 mM NaCl
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
- 别名
- MUSK (MUSK 产品)
- 别名
- unp Protein, fj98e12 Protein, zgc:63467 Protein, wu:fj98e12 Protein, si:dz46m7.2 Protein, si:dz57f23.3 Protein, si:busm1-57f23.3 Protein, musk-A Protein, MuSK Protein, MUSK Protein, Mdk4 Protein, Mlk Protein, Nsk1 Protein, Nsk2 Protein, Nsk3 Protein, muscle Protein, muscle associated receptor tyrosine kinase Protein, muscle, skeletal, receptor tyrosine kinase Protein, muscle associated receptor tyrosine kinase S homeolog Protein, MUSK Protein, musk Protein, musk.S Protein, Musk Protein
- 背景
- Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle (PubMed:25537362). Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation (By similarity). {ECO:0000250, ECO:0000269|PubMed:25537362}.
- 分子量
- 52.8 kDa Including tag.
- UniProt
- O15146
- 途径
- RTK signaling, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development
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