MAP2K4 Protein (AA 2-399) (His tag)
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- 抗原 See all MAP2K4 蛋白
- MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-399
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宿主
- 人
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资源
- Insect Cells
- 标记
- This MAP2K4 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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AAPSPSGGGG SGGGSGSGTP GPVGSPAPGH PAVSSMQGKR KALKLNFANP PFKSTARFTL NPNPTGVQNP HIERLRTHSI ESSGKLKISP EQHWDFTAED LKDLGEIGRG AYGSVNKMVH KPSGQIMAVK RIRSTVDEKE QKQLLMDLDV VMRSSDCPYI VQFYGALFRE GDCWICMELM STSFDKFYKY VYSVLDDVIP EEILGKITLA TVKALNHLKE NLKIIHRDIK PSNILLDRSG NIKLCDFGIS GQLVDSIAKT RDAGCRPYMA PERIDPSASR QGYDVRSDVW SLGITLYELA TGRFPYPKWN SVFDQLTQVV KGDPPQLSNS EEREFSPSFI NFVNLCLTKD ESKRPKYKEL LKHPFILMYE ERAVEVACYV CKILDQMPAT PSSPMYVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MAP2K4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MAP2K4 蛋白
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Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-399) (Active) protein (GST tag)
MAP2K4 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-144) protein (His-IF2DI Tag)MAP2K4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-399) protein (GST tag)MAP2K4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-100) protein (GST tag)MAP2K4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (GST tag,His tag)MAP2K4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 2-397) protein (His tag)Crystallography grade MAP2K4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 2-397) protein (His-SUMO Tag)MAP2K4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (Myc-DYKDDDDK Tag)MAP2K4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))
- 别名
- MAP2K4 (MAP2K4 产品)
- 别名
- JNKK Protein, JNKK1 Protein, MAPKK4 Protein, MEK4 Protein, MKK4 Protein, PRKMK4 Protein, SAPKK-1 Protein, SAPKK1 Protein, SEK1 Protein, SERK1 Protein, SKK1 Protein, Sek1 Protein, Serk1 Protein, MKK4A-l Protein, map2k4 Protein, zgc:136500 Protein, jnkk Protein, mek4 Protein, mkk4 Protein, sek1 Protein, jnkk1 Protein, serk1 Protein, xmek2 Protein, mapkk4 Protein, prkmk4 Protein, 142758_at Protein, CG9738 Protein, CT27508 Protein, D-MKK4 Protein, DMKK4 Protein, Dmel\\CG9738 Protein, JNKK2 Protein, Mpk4 Protein, SEK1/MKK4 Protein, dMKK4 Protein, dMkk4 Protein, hep Protein, ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4 Protein, ATMEK4 Protein, ATMKK4 Protein, F19C24.26 Protein, F19C24_26 Protein, MAP KINASE KINASE 4 Protein, mitogen-activated protein kinase kinase 4 Protein, MKK4B Protein, zgc:194486 Protein, mitogen-activated protein kinase kinase 4 Protein, mitogen-activated protein kinase kinase 4a Protein, MAP kinase kinase 4 Protein, mitogen activated protein kinase kinase 4 Protein, mitogen-activated protein kinase kinase 4b Protein, MAP2K4 Protein, Map2k4 Protein, map2k4a Protein, map2k4 Protein, Mkk4 Protein, MKK4 Protein, map2k4b Protein
- 背景
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. {ECO:0000269|PubMed:7716521}.
- 分子量
- 45.1 kDa Including tag.
- UniProt
- P45985
- 途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
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