EPH Receptor B4 Protein (EPHB4) (AA 561-987) (His tag)
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- 抗原 See all EPH Receptor B4 (EPHB4) 蛋白
- EPH Receptor B4 (EPHB4)
- 蛋白类型
- Recombinant
- 产品特性
- AA 561-987
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宿主
- 人
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资源
- Insect Cells
- 标记
- This EPH Receptor B4 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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LCLRKQSNGR EAEYSDKHGQ YLIGHGTKVY IDPFTYEDPN EAVREFAKEI DVSYVKIEEV IGAGEFGEVC RGRLKAPGKK ESCVAIKTLK GGYTERQRRE FLSEASIMGQ FEHPNIIRLE GVVTNSMPVM ILTEFMENGA LDSFLRLNDG QFTVIQLVGM LRGIASGMRY LAEMSYVHRD LAARNILVNS NLVCKVSDFG LSRFLEENSS DPTYTSSLGG KIPIRWTAPE AIAFRKFTSA SDAWSYGIVM WEVMSFGERP YWDMSNQDVI NAIEQDYRLP PPPDCPTSLH QLMLDCWQKD RNARPRFPQV VSALDKMIRN PASLKIVARE NGGASHPLLD QRQPHYSAFG SVGEWLRAIK MGRYEESFAA AGFGSFELVS QISAEDLLRI GVTLAGHQKK ILASVQHMKS QAKPGTPGGT GGPAPQY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human EPHB4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EPHB4 蛋白
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EPH Receptor B4 (EPHB4) (AA 16-539) protein (His tag)
EPHB4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B4 (EPHB4) (AA 577-987) (Active) protein (GST tag)EPHB4 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
EPH Receptor B4 (EPHB4) (AA 563-987) (Active) protein (His-GST)EPHB4 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
EPH Receptor B4 (EPHB4) (AA 198-323) protein (GST tag)EPHB4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor B4 (EPHB4) (AA 1-987) protein (GST tag)EPHB4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
EPH Receptor B4 (EPHB4) (Active) protein (Fc Tag)EPHB4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
EPH Receptor B4 (EPHB4) (AA 563-987) (Active) protein (GST tag,His tag)EPHB4 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
EPH Receptor B4 (EPHB4) (Active) protein (His tag)EPHB4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
EPH Receptor B4 (EPHB4) (AA 16-539) protein (His tag)Crystallography grade EPHB4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
EPH Receptor B4 (EPHB4) (AA 16-539) protein (His tag)Crystallography grade EPHB4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EPH Receptor B4 (EPHB4)
- 别名
- EPHB4 (EPHB4 产品)
- 别名
- HTK Protein, MYK1 Protein, TYRO11 Protein, htk Protein, myk1 Protein, ephB4a Protein, tyro11 Protein, ephb4b Protein, MGC130997 Protein, AI042935 Protein, Htk Protein, MDK2 Protein, Myk1 Protein, Tyro11 Protein, cb646 Protein, ephB4 Protein, rtk5 Protein, EphB-rtk8 Protein, cb417 Protein, rtk8 Protein, EPH receptor B4 Protein, EPH receptor B4 L homeolog Protein, EPH receptor B4 S homeolog Protein, Eph receptor B4 Protein, eph receptor B4a Protein, eph receptor B4b Protein, EPHB4 Protein, ephb4.L Protein, ephb4.S Protein, ephb4 Protein, Ephb4 Protein, ephb4a Protein, ephb4b Protein
- 背景
- Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. {ECO:0000269|PubMed:12734395, ECO:0000269|PubMed:16424904}.
- 分子量
- 48.6 kDa Including tag.
- UniProt
- P54760
- 途径
- RTK signaling
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