C4B Protein (AA 757-1446) (His tag)
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- 抗原 See all C4B (C4b) 蛋白
- C4B (C4b) (Complement Component C4b (C4b))
- 蛋白类型
- Recombinant
- 产品特性
- AA 757-1446
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宿主
- 人
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资源
- Insect Cells
- 标记
- This C4B protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ALEILQEEDL IDEDDIPVRS FFPENWLWRV ETVDRFQILT LWLPDSLTTW EIHGLSLSKT KGLCVATPVQ LRVFREFHLH LRLPMSVRRF EQLELRPVLY NYLDKNLTVS VHVSPVEGLC LAGGGGLAQQ VLVPAGSARP VAFSVVPTAA TAVSLKVVAR GSFEFPVGDA VSKVLQIEKE GAIHREELVY ELNPLDHRGR TLEIPGNSDP NMIPDGDFNS YVRVTASDPL DTLGSEGALS PGGVASLLRL PRGCGEQTMI YLAPTLAASR YLDKTEQWST LPPETKDHAV DLIQKGYMRI QQFRKADGSY AAWLSRGSST WLTAFVLKVL SLAQEQVGGS PEKLQETSNW LLSQQQADGS FQDLSPVIHR SMQGGLVGND ETVALTAFVT IALHHGLAVF QDEGAEPLKQ RVEASISKAS SFLGEKASAG LLGAHAAAIT AYALTLTKAP ADLRGVAHNN LMAMAQETGD NLYWGSVTGS QSNAVSPTPA PRNPSDPMPQ APALWIETTA YALLHLLLHE GKAEMADQAA AWLTRQGSFQ GGFRSTQDTV IALDALSAYW IASHTTEERG LNVTLSSTGR NGFKSHALQL NNRQIRGLEE ELQFSLGSKI NVKVGGNSKG TLKVLRTYNV LDMKNTTCQD LQIEVTVKGH VEYTMEANED YEDYEYDELP AKDDPDAPLQ PVTPLQLFEG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human C4B, C4B_2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product C4b 蛋白
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Complement Component C4b (C4b) (AA 1454-1744) protein (His tag)
C4b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Complement Component C4b (C4b) (AA 1347-1446) protein (GST tag)C4b 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Complement Component C4b (C4b) (AA 774-873) protein (GST tag)C4b 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Complement Component C4b (C4b) (AA 957-1336) protein (His tag)Crystallography grade C4b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 1454-1744) protein (His tag)Crystallography grade C4b 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 680-756) protein (His tag)Crystallography grade C4b 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 1448-1738) protein (His tag)Crystallography grade C4b 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 678-753) protein (His tag)Crystallography grade C4b 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 20-675) protein (His tag)Crystallography grade C4b 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Complement Component C4b (C4b) (AA 20-673) protein (His tag)Crystallography grade C4b 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- C4B (C4b) (Complement Component C4b (C4b))
- 别名
- C4B, C4B_2 (C4b 产品)
- 背景
- Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens., Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
- 分子量
- 76.4 kDa Including tag.
- UniProt
- P0C0L5
- 途径
- Complement System
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