ABL1 Protein (AA 2-1130) (His tag)
-
- 抗原 See all ABL1 蛋白
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-1130
-
宿主
- 人
-
资源
- Insect Cells
- 标记
- This ABL1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
- 序列
-
LEICLKLVGC KSKKGLSSSS SCYLEEALQR PVASDFEPQG LSEAARWNSK ENLLAGPSEN DPNLFVALYD FVASGDNTLS ITKGEKLRVL GYNHNGEWCE AQTKNGQGWV PSNYITPVNS LEKHSWYHGP VSRNAAEYLL SSGINGSFLV RESESSPGQR SISLRYEGRV YHYRINTASD GKLYVSSESR FNTLAELVHH HSTVADGLIT TLHYPAPKRN KPTVYGVSPN YDKWEMERTD ITMKHKLGGG QYGEVYEGVW KKYSLTVAVK TLKEDTMEVE EFLKEAAVMK EIKHPNLVQL LGVCTREPPF YIITEFMTYG NLLDYLRECN RQEVNAVVLL YMATQISSAM EYLEKKNFIH RDLAARNCLV GENHLVKVAD FGLSRLMTGD TYTAHAGAKF PIKWTAPESL AYNKFSIKSD VWAFGVLLWE IATYGMSPYP GIDLSQVYEL LEKDYRMERP EGCPEKVYEL MRACWQWNPS DRPSFAEIHQ AFETMFQESS ISDEVEKELG KQGVRGAVST LLQAPELPTK TRTSRRAAEH RDTTDVPEMP HSKGQGESDP LDHEPAVSPL LPRKERGPPE GGLNEDERLL PKDKKTNLFS ALIKKKKKTA PTPPKRSSSF REMDGQPERR GAGEEEGRDI SNGALAFTPL DTADPAKSPK PSNGAGVPNG ALRESGGSGF RSPHLWKKSS TLTSSRLATG EEEGGGSSSK RFLRSCSASC VPHGAKDTEW RSVTLPRDLQ STGRQFDSST FGGHKSEKPA LPRKRAGENR SDQVTRGTVT PPPRLVKKNE EAADEVFKDI MESSPGSSPP NLTPKPLRRQ VTVAPASGLP HKEEAGKGSA LGTPAAAEPV TPTSKAGSGA PGGTSKGPAE ESRVRRHKHS SESPGRDKGK LSRLKPAPPP PPAASAGKAG GKPSQSPSQE AAGEAVLGAK TKATSLVDAV NSDAAKPSQP GEGLKKPVLP ATPKPQSAKP SGTPISPAPV PSTLPSASSA LAGDQPSSTA FIPLISTRVS LRKTRQPPER IASGAITKGV VLDSTEALCL AISRNSEQMA SHSAVLEAGK NLYTFCVSYV DSIQQMRNKF AFREAINKLE NNLRELQICP ATAGSGPAAT QDFSKLLSSV KEISDIVQR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human ABL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ABL1 蛋白
-
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Thr315Ile-Mutant) (Active) protein (His tag)
ABL1 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (AA 2-1130) (Active) protein (His tag)ABL1 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Glu255Lys-Mutant) (Active) protein (His tag)ABL1 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Gln252His-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Glu255Lys-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Gly250Glu-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Thr315Ile-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (His396Pro-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Tyr253Phe-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (Phe317Ile-Mutant) (Active) proteinABL1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
- 别名
- ABL1 (ABL1 产品)
- 别名
- ABL Protein, JTK7 Protein, bcr/abl Protein, c-ABL Protein, p150 Protein, v-abl Protein, AI325092 Protein, Abl Protein, E430008G22Rik Protein, c-Abl Protein, ABL proto-oncogene 1, non-receptor tyrosine kinase Protein, c-abl oncogene 1, non-receptor tyrosine kinase Protein, Tyrosine-protein kinase abl-1 Protein, ABL1 Protein, Abl1 Protein, abl-1 Protein, abl1 Protein
- 背景
- Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation), ANXA1 (involved in membrane anchoring), DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling), or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. {ECO:0000269|PubMed:10391250, ECO:0000269|PubMed:11971963, ECO:0000269|PubMed:12379650, ECO:0000269|PubMed:12531427, ECO:0000269|PubMed:12672821, ECO:0000269|PubMed:15031292, ECO:0000269|PubMed:15556646, ECO:0000269|PubMed:15657060, ECO:0000269|PubMed:15886098, ECO:0000269|PubMed:16424036, ECO:0000269|PubMed:16678104, ECO:0000269|PubMed:16943190, ECO:0000269|PubMed:17306540, ECO:0000269|PubMed:17623672, ECO:0000269|PubMed:18328268, ECO:0000269|PubMed:18945674, ECO:0000269|PubMed:19891780, ECO:0000269|PubMed:20357770, ECO:0000269|PubMed:20417104, ECO:0000269|PubMed:9037071, ECO:0000269|PubMed:9144171, ECO:0000269|PubMed:9461559}.
- 分子量
- 123.7 kDa Including tag.
- UniProt
- P00519
- 途径
- Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
-
