WNT1
(Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌(E. Coli)
应用范围
Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human WNT1 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
WNT1
(Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
Xint-1 Protein, Xwnt1 Protein, int-1 Protein, int1 Protein, wnt-1 Protein, wnt1-a Protein, Int-1 Protein, Wnt-1 Protein, sw Protein, swaying Protein, BMND16 Protein, INT1 Protein, OI15 Protein, Int1 Protein, WNT-1 Protein, sb:eu647 Protein, zgc:194464 Protein, zgc:194478 Protein, WNT1 Protein, Wnt family member 1 Protein, Wnt family member 1 L homeolog Protein, wingless-type MMTV integration site family, member 1 Protein, WNT1 Protein, wnt1.L Protein, Wnt1 Protein, wnt1 Protein
背景
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.