MGMT Protein (His tag)
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- 抗原 See all MGMT 蛋白
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This MGMT protein is labelled with His tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human O-6-methylguanine-DNA methyltransferase (MGMT), full length, with N-terminal HIS tag, expressed in E.Coli, 50 μg (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MGMT 蛋白
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the N-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- 别名
- O-6-Methylguanine-Dna Methyltransferase (Mgmt) (MGMT 产品)
- 别名
- AGT Protein, AI267024 Protein, Agat Protein, O-6-methylguanine-DNA methyltransferase Protein, MGMT Protein, Mgmt Protein
- 背景
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]
- 分子量
- 26.2 kDa
- NCBI登录号
- NP_002403
- 途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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