MAPKAP Kinase 3 Protein (Myc-DYKDDDDK Tag)
-
- 抗原 See all MAPKAP Kinase 3 (MAPKAPK3) 蛋白
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This MAPKAP Kinase 3 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
-
- Recombinant human MAPKAP Kinase-3 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- MAPKAPK3 activity verified in a biochemical assay:,MAPKAPK3 activity verified in a biochemical assay:
- Top Product
- Discover our top product MAPKAPK3 蛋白
-
Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 1-382) protein (GST tag)
MAPKAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA, KAA
Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 272-382) protein (GST tag)MAPKAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) protein (GST tag)MAPKAPK3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 1-382) protein (Strep Tag)Crystallography grade MAPKAPK3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 1-384) protein (Strep Tag)Crystallography grade MAPKAPK3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
-
- 应用备注
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
-
The tag is located at the C-terminal.
- 限制
- 仅限研究用
-
- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- 抗原
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
- 别名
- Mapkap Kinase-3 (MAPKAPK3 产品)
- 别名
- 3PK Protein, MAPKAP-K3 Protein, MAPKAP3 Protein, MAPKAPK-3 Protein, MK-3 Protein, AI874665 Protein, MK3 Protein, MapkKapk3 Protein, MAPKAPK3 Protein, mapkapk3 Protein, MGC154360 Protein, 3pk Protein, mapkap3 Protein, zgc:158342 Protein, mitogen-activated protein kinase-activated protein kinase 3 Protein, mitogen-activated protein kinase-activated protein kinase 3 S homeolog Protein, MAPKAPK3 Protein, Mapkapk3 Protein, mapkapk3.S Protein, mapkapk3 Protein
- 背景
- This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein.
- 分子量
- 42.8 kDa
- NCBI登录号
- NP_004626
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
-
