FLT3LG Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all FLT3LG 蛋白
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FLT3LG protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Flt3 ligand protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- FLT-3LG generate dendritic cell differentiation: Fms-like tyrosine kinase 3 ligand (Flt3L) is a nonredundant cytokine required for dendritic cell (DC) homeostasis in lymphoid tissues. FLT3L is commonly used to generate pDCs in vitro. In this experiment, 300,000 unfractioned mouse bone marrow cells were cultured with purified recombinant human FLT-3L (OriGene, ) at indicated concentrations. Total live cells were counted at the day 8.
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Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (Fc Tag)
FLT3LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)FLT3LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) protein (Fc Tag)FLT3LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) protein (mFc-His Tag)FLT3LG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)FLT3LG 宿主: 人 宿主: Hi-5 Cells Recombinant >90 % as determined by SDS-PAGE.
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 28-189) protein (His tag)FLT3LG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-181) protein (His tag)FLT3LG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 1-235) protein (GST tag)FLT3LG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-136) protein (GST tag)FLT3LG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-184) protein (His tag)Crystallography grade FLT3LG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 别名
- Flt3 Ligand (FLT3LG 产品)
- 别名
- Flt3lg Protein, Ly72L Protein, FL Protein, FLT3L Protein, Vegfr3 Protein, fms related tyrosine kinase 3 ligand Protein, FMS-like tyrosine kinase 3 ligand Protein, fms-related tyrosine kinase 4 Protein, fms-related tyrosine kinase 3 ligand Protein, FLT3LG Protein, Flt3l Protein, Flt4 Protein, Flt3lg Protein
- 背景
- Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011].
- 分子量
- 26.2 kDa
- NCBI登录号
- NP_001450
- 途径
- RTK signaling
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