FLT3LG Protein (AA 27-185) (Fc Tag)
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- 抗原 See all FLT3LG 蛋白
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-185
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FLT3LG protein is labelled with Fc Tag.
- 原理
- Human FLT3 Ligand Protein
- 序列
- Thr27-Pro185
- 产品特性
- Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Thr27-Pro185.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. The affinity constant of 35.07 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)
FLT3LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) protein (Fc Tag)FLT3LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) protein (mFc-His Tag)FLT3LG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)FLT3LG 宿主: 人 宿主: Hi-5 Cells Recombinant >90 % as determined by SDS-PAGE.
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 28-189) protein (His tag)FLT3LG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-181) protein (His tag)FLT3LG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 1-235) protein (GST tag)FLT3LG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-136) protein (GST tag)FLT3LG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-184) protein (His tag)Crystallography grade FLT3LG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) protein (His tag)Crystallography grade FLT3LG 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose and 0.01 % tween is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 别名
- FLT3 Ligand (FLT3LG 产品)
- 别名
- Flt3lg Protein, Ly72L Protein, FL Protein, FLT3L Protein, Vegfr3 Protein, fms related tyrosine kinase 3 ligand Protein, FMS-like tyrosine kinase 3 ligand Protein, fms-related tyrosine kinase 4 Protein, fms-related tyrosine kinase 3 ligand Protein, FLT3LG Protein, Flt3l Protein, Flt4 Protein, Flt3lg Protein
- 背景
- Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
- 分子量
- 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result.
- 途径
- RTK signaling
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