DNMT3A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
(1 reference)-
- 抗原 See all DNMT3A 蛋白
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 3
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DNMT3A protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human DNMT3A (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:
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DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 692-912) protein (His-IF2DI Tag)
DNMT3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 803-912) protein (GST tag)DNMT3A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) protein (GST tag)DNMT3A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) protein (Strep Tag)Crystallography grade DNMT3A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-908) protein (Strep Tag)Crystallography grade DNMT3A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 680-902), (partial) protein (His tag)DNMT3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." in: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).
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: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." in: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).
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- 抗原
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- 别名
- Dnmt3a (DNMT3A 产品)
- 别名
- DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein
- 背景
- CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
- 分子量
- 101.7 kDa
- NCBI登录号
- NP_072046
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