DFFA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all DFFA 蛋白
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- 蛋白类型
- Recombinant
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DFFA protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human DFFA / ICAD (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DFFA 蛋白
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DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (His tag)
DFFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (GST tag)DFFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 231-331) protein (GST tag)DFFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (Strep Tag)Crystallography grade DFFA 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (Strep Tag)Crystallography grade DFFA 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- 别名
- Dffa,icad (DFFA 产品)
- 别名
- DFF-45 Protein, DFF1 Protein, ICAD Protein, A330085O09Rik Protein, DFF35 Protein, Dff45 Protein, ICAD-L Protein, ICAD-S Protein, DFFA Protein, zgc:92229 Protein, DNA fragmentation factor subunit alpha Protein, DNA fragmentation factor, alpha subunit Protein, DNA fragmentation factor, 45kDa, alpha polypeptide Protein, DNA fragmentation factor, alpha polypeptide Protein, DFFA Protein, Dffa Protein, dffa Protein
- 背景
- Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- 分子量
- 36.3 kDa
- NCBI登录号
- NP_004392
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
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