AKT2 Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all AKT2 蛋白
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This AKT2 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human AKT2 / PKB beta protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- AKT2 activity verified in a biochemical assay:,AKT2 activity verified in a biochemical assay:
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- Discover our top product AKT2 蛋白
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein
AKT2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant WB
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 107-481) (Active) protein (His-GST)AKT2 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-123) protein (His-IF2DI Tag)AKT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) protein (GST tag)AKT2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (GST tag,His tag)AKT2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-481) protein (Strep Tag)Crystallography grade AKT2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-481) protein (Strep Tag)Crystallography grade AKT2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- Akt2,pkb beta (AKT2 产品)
- 别名
- AKT2 Protein, pkbb Protein, pkbbeta Protein, pkbss Protein, prkbb Protein, rac-beta Protein, v-akt2 Protein, 2410016A19Rik Protein, AW554154 Protein, PKB Protein, PKBbeta Protein, HIHGHH Protein, PKBB Protein, PKBBETA Protein, PRKBB Protein, RAC-BETA Protein, cb945 Protein, akt2-a Protein, AKT serine/threonine kinase 2 Protein, v-akt murine thymoma viral oncogene homolog 2 Protein, thymoma viral proto-oncogene 2 Protein, v-akt murine thymoma viral oncogene homolog 2 L homeolog Protein, AKT2 Protein, akt2 Protein, Akt2 Protein, akt2.L Protein
- 背景
- This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
- 分子量
- 55.6 kDa
- NCBI登录号
- NP_001617
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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