p300 Protein (AA 1041-1161) (His tag,DYKDDDDK Tag)
EP300
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
Bind, ScA
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- 抗原 See all p300 (EP300) 蛋白
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1041-1161
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This p300 protein is labelled with His tag,DYKDDDDK Tag.
- 应用范围
- Binding Studies (Bind), Screening Assay (ScA)
- 产品特性
- The peptide corresponding to amino acids 1041 - 1161 that contains the bromodomain sequences of EP300 (accession number NM_001429.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.1 kDa. It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102.
- Top Product
- Discover our top product EP300 蛋白
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E1A Binding Protein P300 (EP300) (AA 731-830) protein (GST tag)
EP300 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
E1A Binding Protein P300 (EP300) protein (DYKDDDDK Tag)EP300 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- 别名
- p300 (EP300 产品)
- 别名
- KAT3B Protein, RSTS2 Protein, p300 Protein, A430090G16 Protein, A730011L11 Protein, p300 HAT Protein, E1A binding protein p300 Protein, histone acetyltransferase p300 Protein, EP300 Protein, LOC446056 Protein, ep300 Protein, Ep300 Protein
- 背景
- E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
- 分子量
- 19.1 kDa
- 途径
- p53 Pathway, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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