WNT7A Protein (AA 32-349)
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- 抗原 See all WNT7A 蛋白
- WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 32-349
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Multiplex Assay (MA)
- 纯度
- >80 % by SDS-PAGE gel and HPLC analyses.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A) (AA 32-349) protein (His tag)
Crystallography grade WNT7A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A) (AA 32-349) protein (His tag)WNT7A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: The ED50 was determined by its ability to inhibit WNT-3a induced alkaline phosphatase production in MC3T3-E1 cells. The estimated ED50 is 40-60 ng/ml, corresponding to a specific activity of 1.67 - 2.5 x 104 units/mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))
- 别名
- WNT-7a (WNT7A 产品)
- 别名
- wnt7a Protein, AI849442 Protein, Wnt-7a Protein, px Protein, tw Protein, Xwnt-7a Protein, wnt-7a Protein, wnt7a-A Protein, Wnt family member 7A Protein, wingless-type MMTV integration site family, member 7Aa Protein, wingless-type MMTV integration site family, member 7A Protein, wingless-type MMTV integration site family member 7A S homeolog Protein, WNT7A Protein, wnt7aa Protein, Wnt7a Protein, wnt7a.S Protein
- 背景
- The WNT protein family has a highly conserved cystein-rich structure, associated with the extracellular matrix (ECM) with high affinity through binding to heparin sulfate glycoproteins. They are carried intercellularly by lipoproteins and mediate signaling activity by binding to transmembrane Frizzled receptors and low-density-related proteins (LRP). They elicit a variety of responses through canonical or non-canonical (PCP or Ca++) pathways. There are two subtypes of WNT-7 that have been identified, WNT-7a and WNT-7b. WNT-7a is a secreted cell surface signaling protein that controls a set of pathways that act on cell fate determination and tissue patterning during embryogenesis. It is known that WNT-7a can modulate the development of the uterus, cerebellum and limbs, while WNT-7b is important in lung and placental development. The human amino acid sequence of WNT-7a shares high similarity (99.4 % ) with mouse WNT-7a. Signals transduced through WNT-7a and its receptor are found to be critical for female organ development, maintaining levels of female sex hormones and the development of oviduct and uterus. Receptors for WNT-7a include frizzled-5, frizzled-7 and frizzled-10. WNT-7a binds to frizzled-7 to induce signaling through SKT/ mTOR pathway in order to further modulate myofibers in a G-protein dependent PI3K pathway. During muscle cell regeneration, WNT-7a in the ECM along with transiently raised fibronectin levels in the stem cell niche can bind the frizzled-7/Sdc4 receptor complex and synergistically induce symmetric divisions of satellite stem cells. Also, in neurogenesis and chondrogenesis, WNT-7a contributes both in regulating and maintaining the level of lineage differentiation factors required to support apoptosis / cell death through binding of different receptors and interactions with ECM proteins.
- 分子量
- The 318 amino acids recombinant protein has a predicted molecular mass 35.5 kDa. The predicted N-terminal amino acid is Leu.
- 途径
- WNT signaling, Stem Cell Maintenance, Asymmetric Protein Localization
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