CD25 Protein (AA 22-236, C-Term)
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- 抗原 See all CD25 (IL2RA) 蛋白
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 22-236, C-Term
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宿主
- 小鼠
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资源
- CHO Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product IL2RA 蛋白
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Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) protein (His tag)
IL2RA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag,Biotin)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) (Active) protein (His tag)IL2RA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 223-798) proteinIL2RA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Interleukin 2 Receptor, alpha (IL2RA) protein (His tag)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 is 0.2 - 0.6 μg/ml, corresponding to a specific activity of 1.6 - 5.0 x 103 units/mg, as determined by inhibition of M07e cell proliferation induced by human IL-2.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- IL-2R alpha (IL2RA 产品)
- 别名
- CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein
- 背景
- IL-2 was initially identified as a T cell growth factor and plays a key role in the development and homeostasis of Tregs. The IL-2 receptor is a trimeric complex constituted by three different subunits: α (CD25), β (CD122), and common γ (CD132) chains expressed on the surface of activated T cells. Upon activation, immune cells proliferate and CD25 is cleaved from the surface by MMP-2 and MMP-9. Soluble IL-2Rα (sIL-2Rα) binds to IL-2 in vitro, but with a low affinity (kD = 0.03 Mol/l) compared with IL-2Rαβγ complex binding of IL-2 (kD = 10-11 Mol/l). sIL-2Rα acts as an inhibitor of IL-2 induced T cell responses in vitro. Also, sIL-2Rα enhances Th17 responses in vitro and acts early during Th17 development by inhibiting signaling downstream of IL-2R through its ability to sequester local IL-2. sIL-2Rα exacerbates experimental autoimmune encephalomyelitis and enhances generation of Th17 type responses in the periphery and increases infiltration of CD4+ Th1 and Th17 cell subsets into the central nervous system. sIL-2Rα is considered a clinically valuable tool for several diseases. High serum levels of sIL-2Rα have been observed in sarcoidosis and autoimmune diseases like systemic lupus erythematosus and rheumatoid arthritis. Also, sIL-2Rα is elevated in neoplastic disorders. It appears useful in stimulating survival and monitoring therapy of malignant melanoma or nasopharyngeal carcinoma.
- 分子量
- The 230 amino acid recombinant protein has a predicted molecular mass of approximately 26.4 kDa. The DTT-reduced and non-reduced glycosylated protein migrate at approximately 50 - 58 kDa and 40 -55 kDa respectively by SDS-PAGE. The predicted N-terminal am
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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