ICAM1 Protein (AA 28-485, C-Term) (Fc Tag)
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- 抗原 See all ICAM1 蛋白
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-485, C-Term
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宿主
- 小鼠
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资源
- CHO Cells
- 标记
- This ICAM1 protein is labelled with Fc Tag.
- 应用范围
- ELISA, Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 1.0 EU per μg of protein as determine by the LAL method.
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Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-480) protein (His tag)
ICAM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-485) protein (His tag)ICAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-480) protein (His tag)ICAM1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Intercellular Adhesion Molecule 1 (ICAM1) protein (mFc Tag)ICAM1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-480) (Active) protein (Fc Tag)ICAM1 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Intercellular Adhesion Molecule 1 (ICAM1) (AA 26-272) protein (His-IF2DI Tag)ICAM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Intercellular Adhesion Molecule 1 (ICAM1) (AA 1-532) protein (GST tag)ICAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag)ICAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag,Fc Tag)ICAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag)ICAM1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Immobilized mouse ICAM-1-Fc chimera is able to bind retinoic acid (2 μM, 72 hr) activated HL60 cells (5 x 104 /well) in a dose dependent manner. Over 80% of the cells were able to bind mouse ICAM-1 at a dose greater than 12.5 μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 浓度
- 100 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 别名
- ICAM-1 (ICAM1 产品)
- 别名
- BB2 Protein, CD54 Protein, P3.58 Protein, Icam-1 Protein, Ly-47 Protein, MALA-2 Protein, ICAM Protein, ICAM-1 Protein, ICAM1 Protein, intercellular adhesion molecule 1 Protein, intercellular adhesion molecule-1 Protein, ICAM1 Protein, Icam1 Protein, ICAM-1 Protein
- 物质类
- Viral Protein
- 背景
- ICAM-1, intercellular adhesion molecule 1, is a member of the immunoglobulin superfamily. Structurely, ICAM-1 is characterized by heavy glycosylation with an extracellular domain composed of multiple loops created by disulfide bridges within the protein. The dominant secondary structure of the protein is the beta sheet, leading investigators to hypothesize the presence of dimerization domains within ICAM-1. This protein is constitutively expressed at low levels on the cell surface of leukocytes, endothelial cells, macrophages, and lymphocytes. Upon cytokine activation, by cytokines such as IL-1 and TNFα, the concentration of ICAM-1 on the cell surface greatly increases. The ligand of ICAM-1 is LFA-1 (leukocyte function associated antigen 1), which is found on all T cells. LFA-1/ICAM-1 interaction has been shown to be important for T cell-T cell interactions, which leads to further T cell differentiation. ICAM-1 was found to play a role in infectious diseases, as it was identified as the binding target for entry of the major group of human rhinoviruses into various cell types and is also known for its affinity for Plasmodium falciparum-infected erythrocytes. These findings indicate that ICAM-1 may participate in the pathogenesis of several infectious agents
- 分子量
- The 696 amino acid recombinant protein, with a C-terminal human IgG1 Fc tag, has a predicted molecular mass of approximately 77 kDa. The protein migrates near 130 kDa by SDS-PAGE in DTT-reducing conditions and near 250 kDa in non-reducing conditions. The
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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