IL-27 Protein (AA 1-16, AA 19-228, AA 29-234)
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- 抗原 See all IL-27 (IL27) 蛋白
- IL-27 (IL27) (Interleukin 27 (IL27))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-16, AA 19-228, AA 29-234
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 纯度
- Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
IL27 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) (AA 177-243) protein (GST tag)IL27 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) protein (His tag)IL27 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Interleukin 27 (IL27) proteinIL27 宿主: 小鼠 宿主: 大肠杆菌（E. Coli）
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 = 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 105 units/mg, as determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in 20 mM NaH2PO4, pH 6, 0.60 M NaCl.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- IL-27 (IL27) (Interleukin 27 (IL27))
- 别名
- IL-27 (IL27 产品)
- 别名
- IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein
- 背景
- IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related polypeptide. IL-27 can be allocated to the IL-6/IL-12 superfamily of cytokines which include also IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common four-helix bundle, their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1 and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27-specific receptor chain, gp130 is the common receptor subunit of IL-6 type cytokines. IL-27 signaling in naive CD4+ T cells leads to activation and phosphorylation of STAT1 and STAT3.
- 分子量
- The total predicted molecular weight is 49 kDa. The non-reduced and the DTT-reduced proteins migrate as one band at approximately 55 kDa by SDS-PAGE.
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