IL-27 Protein (AA 21-229) (His tag)
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- 抗原 See all IL-27 (IL27) 蛋白
- IL-27 (IL27) (Interleukin 27 (IL27))
- 蛋白类型
- Recombinant
- 产品特性
- AA 21-229
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宿主
- 人
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资源
- CHO Cells
- 标记
- This IL-27 protein is labelled with His tag.
- 原理
- Human IL-27 Protein
- 序列
- Arg21-Lys229 (IL-27B) & Phe29-Pro243 (IL-27A)
- 特异性
- Uni-Prot: Q14213 (IL-27B), Q8NEV9 (IL-27A)
- 产品特性
- Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A).
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product IL27 蛋白
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
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Interleukin 27 (IL27) (AA 29-243) protein (His tag)Crystallography grade IL27 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 27 (IL27) (AA 29-234) protein (His tag)Crystallography grade IL27 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-27 (IL27) (Interleukin 27 (IL27))
- 别名
- IL-27 (IL27 产品)
- 别名
- IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein
- 背景
- Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
- 分子量
- 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
- UniProt
- Q14213
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