RANKL Protein (AA 158-316)
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- 抗原 See all RANKL (TNFSF11) 蛋白
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 158-316
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宿主
- 小鼠
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资源
- Insect Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product TNFSF11 蛋白
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Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 63-244) protein (His-DYKDDDDK Tag)
TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 63-244) proteinTNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70-316) protein (His tag)TNFSF11 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 158-317) protein (His-DYKDDDDK-Strep II Tag)TNFSF11 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70-244) proteinTNFSF11 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant WB, Imm
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70 – 244) protein (His tag)TNFSF11 宿主: 人 Recombinant > 97 % by SDS-PAGE gel WB, Imm, CC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (Fc Tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) protein (Fc Tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (His tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (AVI tag,Fc Tag,Biotin)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Bioactivity was measured by its property to induce osteoclast differentiation in RAW264.7 cells in the absence of any cross-linking. The bioactivity is equivalent to competitor cytokines.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- 别名
- TRANCE (TNFSF11 产品)
- 别名
- CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, TNFSF11 Protein, Ly109l Protein, OPG Protein, Trance Protein, cd254 Protein, odf Protein, opgl Protein, optb2 Protein, rankl Protein, sodf Protein, tnfsf11 Protein, trance Protein, TNF superfamily member 11 Protein, tumor necrosis factor (ligand) superfamily, member 11 Protein, TNF receptor superfamily member 11b Protein, TNF superfamily member 11 L homeolog Protein, TNFSF11 Protein, Tnfsf11 Protein, TNFRSF11B Protein, tnfsf11.L Protein
- 背景
- RANKL gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kD. RANKL is cleaved to produce a soluble form with biological activity. The shedding of membrane-bound RANKL appears to be mediated by expression of matrix metalloproteinase (MMP) 14 and ADAM10. Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore, RANKL shedding seems to be an important process that down-regulates local osteoclastogenesis. Alternatively, an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation, activation, and survival, which results in increased bone resorption. Binding of RANKL to its receptor RANK activates TNF receptor- associated factor 6 (TRAF6), which is linked to downstream pathways including NF-kB, c-jun N-terminal kinase (JNK) or Src. TRAF6 has been shown to be necessary for the differentiation of osteoclastic cells by enhancing Src kinase, essential for osteoclast function.
- 分子量
- The 178 amino acid recombinant protein has a predicted molecular mass of approximately 20 kDa. The DTT-reduced protein migrates at approximately 20 kDa by SDS-PAGE.
- 途径
- NF-kappaB Signaling
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