RANKL Protein (AA 63-244) (His-DYKDDDDK Tag)
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- 抗原 See all RANKL (TNFSF11) 蛋白
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- 蛋白类型
- Recombinant
- 产品特性
- AA 63-244
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This RANKL protein is labelled with His-DYKDDDDK Tag.
- 原理
- Human RANKL/TNFSF11/CD254 Protein
- 序列
- Gly63-Asp244
- 产品特性
- Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244.
- 纯度
- > 90 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human RANKL, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 63-244) protein
TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70-316) protein (His tag)TNFSF11 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 158-317) protein (His-DYKDDDDK-Strep II Tag)TNFSF11 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70-244) proteinTNFSF11 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant WB, Imm
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70 – 244) protein (His tag)TNFSF11 宿主: 人 Recombinant > 97 % by SDS-PAGE gel WB, Imm, CC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (Fc Tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) protein (Fc Tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (His tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (AVI tag,Fc Tag,Biotin)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (His tag,AVI tag,Biotin)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
- 别名
- RANKL (TNFSF11 产品)
- 别名
- CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, TNFSF11 Protein, Ly109l Protein, OPG Protein, Trance Protein, cd254 Protein, odf Protein, opgl Protein, optb2 Protein, rankl Protein, sodf Protein, tnfsf11 Protein, trance Protein, TNF superfamily member 11 Protein, tumor necrosis factor (ligand) superfamily, member 11 Protein, TNF receptor superfamily member 11b Protein, TNF superfamily member 11 L homeolog Protein, TNFSF11 Protein, Tnfsf11 Protein, TNFRSF11B Protein, tnfsf11.L Protein
- 背景
- Receptor activator of nuclear factor x03BA,B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
- 分子量
- 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result.
- 途径
- NF-kappaB Signaling
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