TNFSF14 Protein (AA 72-239, N-Term)
-
- 抗原 See all TNFSF14 蛋白
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 72-239, N-Term
-
宿主
- 小鼠
-
资源
- CHO Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
-
Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product TNFSF14 蛋白
-
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (Trimer) protein (His-DYKDDDDK Tag)
TNFSF14 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 74-240) protein (His tag)TNFSF14 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 74-240) protein (Fc Tag)TNFSF14 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 89-240) protein (His-DYKDDDDK-Strep II Tag)TNFSF14 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 74-240) (Active) protein (His tag)TNFSF14 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 71-240) protein (His-IF2DI Tag)TNFSF14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 1-177) protein (GST tag)TNFSF14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 1-204) protein (GST tag)TNFSF14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 61-170) protein (GST tag)TNFSF14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: ED50 = 1.0 - 5.0 μg/mL, as determined by cytotoxicity effect of HTâ€'29 human colon adenocarcinoma cells in the presence of human IFN-γ.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
-
- 抗原
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- 别名
- LIGHT (TNFSF14 产品)
- 别名
- TNFSF14 Protein, CD258 Protein, HVEML Protein, LIGHT Protein, LTg Protein, TR2 Protein, HVEM-L Protein, Ly113 Protein, TNF superfamily member 14 Protein, tumor necrosis factor (ligand) superfamily, member 14 Protein, TNFSF14 Protein, Tnfsf14 Protein
- 背景
- LIGHT is a member of the TNF family which includes TNF-α, TNF-β (lymphotoxin-alpha, LT-α), CD40 ligand, TRAIL (Apo2L), Fas ligand (FASL), and osteoprotegerin (OPG) ligand. LIGHT is a type II transmembranal protein that possesses 34 % identity with lymphotoxin beta (LT-β) and 31 % identity with FASL. LIGHT stimulates the proliferation of activated HVEM+ T-cells, NF-κB activation, and induces apoptosis in cells expressing both HVEM and LT-β receptors. B- and T- lymphocyte attenuators (BTLA) also bind to HVEM. LIGHT, as well as other members of the TNF/TNFR family, is released from the membrane by MMPs. It plays a key role for optimal IL-12 production by the dendritic cells (DCs) and the development of IFN-γ-producing CD4+ Th1 cells. Bone marrow-derived DCs from LIGHT (-/-) mice are severely impaired in IL-12p40 production after IFN-γ and LPS stimulation in vitro. LIGHT is expressed in lung inflammatory leucocytes after an allergen house dust mite challenge. It induces airway remodeling via the induction of TGF-β. The inhibition of LIGHT, using a fusion protein of Fc with the extracellular portion of the LT-β receptor (LTβR-Fc), prevents LIGHT-LTβR and LIGHT-HVEM interaction. In a mouse model of chronic asthma, this reduces lung fibrosis, smooth muscle and epithelial hyperplasia, and airway hyperresponsiveness through the suppression of lung TGF-β and IL-13 production. LIGHT has been shown to increase the anti-tumor immune response and its overexpression is associated with the survival in patients with colorectal cancer liver metastasis.
- 分子量
- The 189 amino acid recombinant protein has a predicted molecular mass of approximately 20.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 30 and 28 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
- 途径
- Cancer Immune Checkpoints
-
