TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
蛋白类型
Recombinant
产品特性
AA 74-240
宿主
人
资源
HEK-293 Cells
标记
This TNFSF14 protein is labelled with His tag.
原理
Human LIGHT/TNFSF14 Protein
序列
Asp74-Val240
产品特性
Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asp74-Val240.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LIGHT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Crystallography grade
TNFSF14
宿主: 小鼠
宿主: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
分子量
19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.