GZMA Protein (AA 26-262)
-
- 抗原 See all GZMA 蛋白
- GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 26-262
-
宿主
- 人
-
资源
- HEK-293 Cells
- 应用范围
- Intracellular Flow Cytometry (ICFC)
- 纯度
- > 90 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
-
Less than 1.0 EU per μg of protein as determine by the LAL method.
- Top Product
- Discover our top product GZMA 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: Lysyl endopeptidase activated human granzyme A cleaves the peptide substrate N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >5,000 pmol/min/μg.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.4.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -70°C for six months.
-
- 抗原
- GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))
- 别名
- Granzyme A (GZMA 产品)
- 别名
- gzmA Protein, GZMA Protein, AW494114 Protein, Ctla-3 Protein, Ctla3 Protein, Hf Protein, SE1 Protein, TSP-1 Protein, TSP1 Protein, CTLA3 Protein, HFSP Protein, granzyme A Protein, Granzyme A Protein, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) Protein, gzmA Protein, GZMA Protein, graa Protein, Gzma Protein
- 背景
- Granzyme A is a serine protease belonging to the granzyme family and is expressed exclusively by cytotoxic T cells (CTL) and NK cells. Most circulating CD56+CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzymes A and B. In contrast, few circulating CD4+ T cells express granzyme A or B. Activation of CD8+ and CD4+ T lymphocytes induces substantial expression of granzyme B, but not granzyme A. Following receptor-mediated conjugate formation between a granzyme-containing cell and an infected or transformed target cell, granzymes enter the target cell via endocytosis and induce apoptosis. Granzyme A was found to induce caspase independent cell death when it enters into the target cell by perforin. Once in a cell, granzyme A activates DNA nicking by DNAse NM23-H1, a tumor suppressor gene product whose expression is reduced in transformed, metastatic cells. Dysregulation of this pathway results in several human diseases, such as hemophagocytic lymphohistiocytosis. Besides the protease activity, granzyme A induces human lung fibroblasts to produce IL-6 and IL-8. Cytokine induction is abrogated by treating the serine protease with the suicide serine protease inhibitor 3,4-dichloroisocoumarin. Other fibroblast lines, as well as epithelial cells, produce cytokines in response to granzyme A. These findings suggest that granzyme A can function as an activation molecule with potentially important immunoregulatory functions. However, CTLs from mice lacking granzyme A induce morphologically normal apoptosis in vitro, but those from mice that are deficient of granzyme B induce the nuclear features of apoptosis (particularly DNA fragmentation) more slowly than do wild-type CTLs.
- 分子量
- This 252 amino acid recombinant protein has a predicted molecular mass of approximately 28 kDa. The protein migrates at about 35 kDa in DTT-reducing conditions and about 55 kDa in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is
- 途径
- Apoptosis
-