Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/mL of recombinant IL-2, ED50 for this effect is < 1.5 μg/mL. AA 22-213, expressed with N-terminal 8xHis
Crystallography grade
IL2RA
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O or PBS at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant Human Interleukin-2 Receptor alpha remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, Human Interleukin-2 Receptor alpha should be stable up to 1 week at 4 °C or up to 3 months at -20 °C.
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. The IL-2R is made up of 3 subunits - alpha , beta and gamma . The alpha and beta chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the gamma-chain, along with the beta subunit. The beta and gamma chains of the IL-2R are members of the type I cytokine receptor family. IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). IL-2 R alpha is also known as CD25, p55, and Tac (activated T cell) antigen.Recombinant Human Interleukin-2 Receptor alpha (IL-2 R alpha), His produced in HEK 293 cells is a polypeptide chain containing 205 amino acids. A fully biologically active molecule, rhIL-2 R alpha has a molecular mass of 42 kDa analyzed by reducing SDS-PAGE. Synonyms: soluble IL-2 receptor, TAC-antigen, CD25 antigen