CCL11 蛋白
-
- 抗原 See all CCL11 products
- CCL11 (Chemokine (C-C Motif) Ligand 11 (CCL11))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 序列
- GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP
- 产品特性
- Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/mL.
- 纯度
- > 97 % by SDS-PAGE and HPLC analyses.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- < 1 EU/μg of rHuEotaxin/CCL11 as determined by LAL method.
-
Chemokine (C-C Motif) Ligand 11 (CCL11) (AA 24-97) protein (His tag)
CCL11 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Chemokine (C-C Motif) Ligand 11 (CCL11) (AA 24-97) protein (His tag)CCL11 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Chemokine (C-C Motif) Ligand 11 (CCL11) (AA 24-97) protein (His tag)CCL11 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- 缓冲液
- Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
-
- 抗原
- CCL11 (Chemokine (C-C Motif) Ligand 11 (CCL11))
- 别名
- Eotaxin/CCL11 (CCL11 产品)
- 别名
- SCYA11 Protein, Scya11 Protein, eotaxin Protein, Eotaxin Protein, C-C motif chemokine ligand 11 Protein, chemokine (C-C motif) ligand 11 Protein, CCL11 Protein, Ccl11 Protein
- 背景
-
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60 % identical to mature mouse and guinea pig CCL11. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.
Synonyms: Eotaxin/CCL11 , Human, - 分子量
- 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
- 途径
- Regulation of Actin Filament Polymerization, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-
