Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor/MANF is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (AA 25-182) of Human MANF fused with a polyhistidine tag at the C-termin
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
MANF also called ARMET or ARP, is short for Mesencephalic astrocyte- derived neurotrophic factor. It is a 182 aa. protein with a 24 aa. signal peptide. The N-terminal region of MANF may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. It is secreted and can be inducted by endoplasmic reticulum stress. This protein selectively promotes the survival of dopaminergic neurons of the ventral mid-brain, and can modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. It also enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons and inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.