MANF Protein (His tag)
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- 抗原 See all MANF 蛋白
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 人细胞
- 标记
- This MANF protein is labelled with His tag.
- 原理
- Recombinant Human MANF/ARMET Protein (His Tag)
- 序列
- Leu25-Leu182
- 产品特性
- Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor is produced by our Mammalian expression system and the target gene encoding Leu25-Leu182 is expressed with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product MANF 蛋白
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Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Active) protein
MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) protein (GST tag)MANF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 116-185) protein (GST tag)MANF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (His tag)MANF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)Crystallography grade MANF 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 22-179) protein (His tag)Crystallography grade MANF 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- 别名
- MANF/ARMET (MANF 产品)
- 别名
- ARMET Protein, ARP Protein, 3230402M22Rik Protein, AA407711 Protein, AA408789 Protein, AA673178 Protein, Armet Protein, D17914 Protein, D18Mgi17 Protein, MANF Protein, RBM15B Protein, ARP-like Protein, CG7013 Protein, DmMANF Protein, Dmel\\CG7013 Protein, anon-EST:ParkEST188 Protein, arp Protein, armet Protein, im:6909481 Protein, zgc:153327 Protein, manf Protein, mesencephalic astrocyte derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor Protein, RNA binding motif protein 15B Protein, Mesencephalic astrocyte-derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor S homeolog Protein, MANF Protein, Manf Protein, RBM15B Protein, manf Protein, manf.S Protein
- 背景
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Background: Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. MANF enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF and a homologous protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. MANF expression was widespread in the nervous system and non-neuronal tissues.
Synonym: Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET, ARMET, ARP
- 分子量
- 19.2 kDa
- UniProt
- P55145
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