RHEB Protein (AA 1-184) (GST tag)
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- 抗原 See all RHEB 蛋白
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-184
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This RHEB protein is labelled with GST tag.
- 原理
- Recombinant Human GTP-Binding Protein Rheb/RHEB (N-GST)
- 序列
- MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF RRIILEAEKM DGAASQGKSS CSVM
- 产品特性
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Recombinant Human GTP-Binding Protein Rheb/RHEB (N-GST)
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product RHEB 蛋白
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Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (T7 tag)
RHEB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (His tag)RHEB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (GST tag)RHEB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (His tag)Crystallography grade RHEB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (His tag)Crystallography grade RHEB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Ras Homolog Enriched in Brain (RHEB) protein (Myc-DYKDDDDK Tag)RHEB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 10 mM GSH, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- 别名
- GTP-Binding Protein Rheb/RHEB (RHEB 产品)
- 别名
- RHEB2 Protein, RHEB Protein, rheb2 Protein, CG1081 Protein, DReb Protein, Dm Rheb Protein, DmRheb Protein, Dmel\CG1081 Protein, Q9VND8 Protein, RheB Protein, dRheb Protein, rheb Protein, Ras homolog, mTORC1 binding Protein, Ras homolog enriched in brain Protein, ras homolog enriched in brain Protein, Ras homolog, mTORC1 binding L homeolog Protein, RHEB Protein, Rheb Protein, rheb Protein, rheb.L Protein
- 背景
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Recombinant Human GTP-Binding Protein Rheb/RHEB is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met184) of Human RHEB fused with a GST tag at the N-terminus.
GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle, and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling, and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, farnesylation of the protein is required for this activity. - 分子量
- 20.4 kDa
- UniProt
- Q15382
- 途径
- RTK signaling
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