APEX1 Protein (AA 1-318) (GST tag)
(1 reference)-
- 抗原 See all APEX1 蛋白
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-318
-
宿主
- 人
-
资源
- 小麦胚
- 标记
- This APEX1 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- 原理
- APEX1 (Human) Recombinant Protein (P01)
- 序列
- MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD QKTSPSGKPA TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS ENKLPAELQE LPGLSHQYWS APSDKEGYSG VGLLSRQCPL KVSYGIGEEE HDQEGRVIVA EFDSFVLVTA YVPNAGRGLV RLEYRQRWDE AFRKFLKGLA SRKPLVLCGD LNVAHEEIDL RNPKGNKKNA GFTPQERQGF GELLQAVPLA DSFRHLYPNT PYAYTFWTYM MNARSKNVGW RLDYFLLSHS LLPALCDSKI RSKALGSDHC PITLYLAL
- 产品特性
- Human APEX1 full-length ORF ( AAH02338, 1 a.a. - 318 a.a.) recombinant protein with GST-tag at N-terminal.
- 纯化方法
- in vitro wheat germ expression system
-
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 97-195) protein (His-IF2DI Tag)
APEX1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) protein (T7 tag)APEX1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) protein (His tag)APEX1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 219-318) protein (GST tag)APEX1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 2-318) protein (His tag)Crystallography grade APEX1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 32-318) protein (His tag)Crystallography grade APEX1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 2-317) protein (His tag)Crystallography grade APEX1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 31-317) protein (His tag)Crystallography grade APEX1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
-
- 缓冲液
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Best use within three months from the date of receipt of this protein.
-
-
: "Bcl2 inhibits abasic site repair by down-regulating APE1 endonuclease activity." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 9925-32, (2008) (PubMed).
-
: "Bcl2 inhibits abasic site repair by down-regulating APE1 endonuclease activity." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 9925-32, (2008) (PubMed).
-
- 抗原
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 别名
- APEX1 (APEX1 产品)
- 别名
- apex1 Protein, MGC76280 Protein, MGC79645 Protein, MGC78928 Protein, APE Protein, APE1 Protein, APEN Protein, APEX Protein, APX Protein, HAP1 Protein, REF1 Protein, Apex Protein, REF-1 Protein, BAP1 Protein, apex1a Protein, hm:zehn1532 Protein, wu:fc13d11 Protein, zgc:66204 Protein, Ref-1 Protein, apurinic/apyrimidinic endodeoxyribonuclease 1 Protein, APEX nuclease (multifunctional DNA repair enzyme) 1 Protein, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog Protein, DNA-(apurinic or apyrimidinic site) lyase Protein, apurinic/apyrimidinic endonuclease 1 Protein, APEX1 Protein, apex1 Protein, apex1.L Protein, CNG03640 Protein, CNH01280 Protein, nth Protein, Spirs_4119 Protein, Palpr_0103 Protein, Apex1 Protein
- 背景
-
Full Gene Name: APEX nuclease (multifunctional DNA repair enzyme) 1
Synonyms: APE,APE-1,APE1,APEN,APEX,APX,HAP1,REF-1,REF1 - 基因ID
- 328
- 途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
-
