HIF1A Protein (AA 1-735) (GST tag)
(3 references)-
- 抗原 See all HIF1A 蛋白
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-735
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宿主
- 人
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资源
- 小麦胚
- 标记
- This HIF1A protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- 原理
- HIF1A (Human) Recombinant Protein (P01)
- 序列
- MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMKAQMNC FYLKALDGFV MVLTDDGDMI YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH RNGLVKKGKE QNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL IFSLQQTECV LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL APAAGDTIIS LDFGSNDTET DDQQLEEVPL YNDVMLPSPN EKLQNINLAM SPLPTAETPK PLRSSADPAL NQEVALKLEP NPESLELSFT MPQIQDQTPS PSDGSTRQSS PEPNSPSEYC FYVDSDMVNE FKLELVEKLF AEDTEAKNPF STQDTDLDLE MLAPYIPMDD DFQLRSFDQL SPLESSSASP ESASPQSTVT VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE TTSATSSPYR DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT VPEEELNPKI LALQNAQRKR KMEHDGSLFQ AVGII
- 产品特性
- Human HIF1A full-length ORF ( NP_851397.1, 1 a.a. - 735 a.a.) recombinant protein with GST-tag at N-terminal.
- 纯化方法
- in vitro wheat germ expression system
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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 576-785) protein (His tag)
HIF1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 575-825) protein (His tag)HIF1A 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 574-799) protein (His tag)HIF1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 717-826) protein (GST tag)HIF1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 1-85) protein (His tag)HIF1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS – PAGE SDS
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 1-826) protein (Strep Tag)Crystallography grade HIF1A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 1-836) protein (Strep Tag)Crystallography grade HIF1A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
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- 缓冲液
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Best use within three months from the date of receipt of this protein.
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: "Hypoxia-inducible factors promote alveolar development and regeneration." in: American journal of respiratory cell and molecular biology, Vol. 50, Issue 1, pp. 96-105, (2014) (PubMed).
: "A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA." in: Analytica chimica acta, Vol. 838, pp. 31-6, (2014) (PubMed).
: "The E3 ubiquitin ligase Pellino3 protects against obesity-induced inflammation and insulin resistance." in: Immunity, Vol. 41, Issue 6, pp. 973-87, (2014) (PubMed).
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: "Hypoxia-inducible factors promote alveolar development and regeneration." in: American journal of respiratory cell and molecular biology, Vol. 50, Issue 1, pp. 96-105, (2014) (PubMed).
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- 抗原
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- 别名
- HIF1A (HIF1A 产品)
- 别名
- hif1a Protein, AA959795 Protein, HIF1alpha Protein, MOP1 Protein, bHLHe78 Protein, HIF-1A Protein, HIF-1alpha Protein, HIF1 Protein, HIF1-ALPHA Protein, PASD8 Protein, HIF1-alpha Protein, hif-1a Protein, hypoxia inducible factor 1 alpha Protein, hypoxia inducible factor 1, alpha subunit Protein, hypoxia inducible factor 1 alpha subunit Protein, HIF (Hypoxia Inducible Factor) homologa Protein, hif-1a Protein, Hif1a Protein, HIF1A Protein, hif1a Protein, hif-1 Protein
- 背景
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Full Gene Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms: HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78 - 基因ID
- 3091
- NCBI登录号
- NM_181054
- 途径
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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