(5 references)-
- 抗原 See all CCL2 蛋白
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 蛋白类型
- Recombinant
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func), Blocking Reagent (BR), Intracellular Flow Cytometry (ICFC)
- 品牌
- BD Pharmingen™
- 特异性
- Upon initial thawing, recombinant rat MCP-1 (MN 555110) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- 产品特性
- Recombinant rat MCP-1 (MN 555110) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing glycerol and 1.0 mg/ml bovine serum albumin, with no preservatives. Recombinant rat MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant rat MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and 1.0 mg/mL bovine serum albumin, with no preservatives. Recombinant rat MCP-1 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of rat MCP-1, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product CCL2 蛋白
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Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His-Avi Tag,Biotin)
CCL2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His-Avi Tag)CCL2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) protein (His tag)CCL2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) (Active) protein (Biotin)CCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA, FACS Active
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) (Active) proteinCCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 1-99) protein (GST tag)CCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) protein (GST tag)CCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His tag)CCL2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-96), (partial) protein (His tag)CCL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
Chemokine (C-C Motif) Ligand 2 (CCL2) proteinCCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant
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- 应用备注
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ELISA Standard: Recombinant rat MCP-1 (MN 555110) can be useful as a quantitative standard for measuring rat MCP-1 protein levels using sandwich ELISA with the purified C4 antibody (Cat. No. 555072) as a capture antibody and the biotinylated B4 antibody (Cat. No. 555074) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant rat MCP-1 from 2000-5 pg/mL to be included for each ELISA plate. For measuring rat MCP-1 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Rat MCP-1 Set (Cat. No. 555130).
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range encompassing an ED50 = 60 - 600 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay. Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant rat MCP-1 can be used as a blocking control to demonstrate the specificity of MCP-1 staining by the PE-conjugated format (Cat. No.554443) of the 2H5 Anti-Rat MCP-1 antibody. Investigators are advised that this blocking application is not routinely tested for this material. The use of staining controls for the immunofluorescent staining and flow cytometric analysis of cytokine producing cells has been previously described (Prussin et al.). - 说明
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BD Pharmingen™ Recombinant Rat MCP-1 - Reactivity Rat
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 mg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
- 储存液
- Azide free
- 注意事项
- Rat Monocyte Chemoattractant Protein (MCP-1) is a member of the C-C chemokine superfamily and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells.3 MCP-1 has chemoattractant activity for monocytes, lymphocytes and basophils but is not active for neutrophils. Recombinant rat MCP-1 is a highly glycosylated protein ranging in size from 27 - 30 kD as measured by SDS-PAGE analysis. Furthermore, there is evidence from experimental animal models that MCP-1 can suppress tumor formation by attracting monocytes to the tumor site.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Leukocyte migration and activation by murine chemokines." in: Immunobiology, Vol. 195, Issue 4-5, pp. 499-521, (1997) (PubMed).
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Suppression of tumor formation in vivo by expression of the JE gene in malignant cells." in: Molecular and cellular biology, Vol. 11, Issue 6, pp. 3125-31, (1991) (PubMed).
: "Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines." in: Biochemical and biophysical research communications, Vol. 174, Issue 2, pp. 504-9, (1991) (PubMed).
: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." in: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).
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: "Leukocyte migration and activation by murine chemokines." in: Immunobiology, Vol. 195, Issue 4-5, pp. 499-521, (1997) (PubMed).
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- 抗原
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 别名
- MCP-1 (CCL2 产品)
- 别名
- GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein
- 背景
- Rat Monocyte Chemoattractant Protein (MCP-1) is a member of the C-C chemokine superfamily and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells.3 MCP-1 has chemoattractant activity for monocytes, lymphocytes and basophils but is not active for neutrophils. Recombinant rat MCP-1 is a highly glycosylated protein ranging in size from 27 - 30 kD as measured by SDS-PAGE analysis. Furthermore, there is evidence from experimental animal models that MCP-1 can suppress tumor formation by attracting monocytes to the tumor site.
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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