Recombinant Human Serine/Threonine-Protein Phosphatase PP1-gamma Catalytic Subunit/PPP1CC is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Lys323) of Human PPP1CC fused with a 6His tag at both the N-terminus and C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
PPM1G
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Liquid
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, pH 8.0 .
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
AI385652 Protein, Fin13 Protein, PP2CG Protein, PP2CGAMMA Protein, PPP2CG Protein, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent 1G Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1G Protein, Ppm1g Protein, PPM1G Protein
Sub Type
Fusionprotein
背景
Serine/Threonine-Protein Phosphatase PP1-Ypsilon Catalytic Subunit (PPP1CC) is a member of the PPP phosphatase family. It is essential for cell division, participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CC colocalizes with SPZ1 in the nucleus, with URI1 at mitochondrion, Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. As a cofactor, PPP1CC binds one iron ion and one manganese ion per subunit.. In addition, PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Alternative Names: Serine/Threonine-Protein Phosphatase PP1-Gamma Catalytic Subunit, PP-1G, Protein Phosphatase 1C Catalytic Subunit, PPP1CC