FEN1 Protein (AA 1-380)
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- 抗原 See all FEN1 蛋白
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-380
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human Flap Endonuclease 1/FEN-1
- 序列
- MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
- 产品特性
- Recombinant Human Flap Endonuclease 1/FEN-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys380) of Human FEN-1.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product FEN1 蛋白
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Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-110) protein (GST tag)
FEN1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (GST tag)FEN1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) protein (Myc-DYKDDDDK Tag)FEN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10 % Glycerol, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- 别名
- flap-endonuclease-1 (FEN1 产品)
- 别名
- FEN-1 Protein, FEN1 Protein, AW538437 Protein, MF1 Protein, RAD2 Protein, cb879 Protein, fen-1 Protein, fen1 Protein, mf1 Protein, rad2 Protein, xFEN1a Protein, flap structure-specific endonuclease 1 Protein, flap endonuclease 1-like Protein, Flap endonuclease-1 Protein, 5'-3' exonuclease family protein Protein, flap structure specific endonuclease 1 Protein, flap structure-specific endonuclease 1 L homeolog Protein, nuclease, Rad2 family Protein, FEN1 Protein, LOC100808044 Protein, Bm1_49605 Protein, AT5G26680 Protein, Fen1 Protein, fen1 Protein, fen1.L Protein
- 背景
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Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Alternative Names: Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2 - 分子量
- 42.59 kDa
- UniProt
- P39748
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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