CDK2 Protein (AA 1-298) (His tag)
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- 抗原 See all CDK2 蛋白
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-298
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CDK2 protein is labelled with His tag.
- 原理
- Recombinant Human Cyclin-Dependent Kinase 2/CDK2 (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL
- 产品特性
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Recombinant Human Cyclin-Dependent Kinase 2/CDK2 (N-6His)
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CDK2 蛋白
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Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) protein (GST tag)
CDK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) (Active) protein (GST tag)CDK2 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-198) protein (His tag)CDK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cyclin-Dependent Kinase 2 (CDK2) (AA 211-298) protein (GST tag)CDK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-346) protein (Strep Tag)Crystallography grade CDK2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- 别名
- Cyclin-Dependent Kinase 2/CDK2 (CDK2 产品)
- 别名
- p33(CDK2) Protein, A630093N05Rik Protein, wu:fa10c02 Protein, zgc:56598 Protein, zgc:77684 Protein, eg1 Protein, CDKN2 Protein, cdk2L Protein, cyclin dependent kinase 2 Protein, cyclin-dependent kinase 2 Protein, cyclin-dependent kinase 2 S homeolog Protein, CDK2 Protein, Cdk2 Protein, cdk2 Protein, cdk2.S Protein
- 背景
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Recombinant Human Cyclin-Dependent Kinase 2/CDK2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu298) of Human CDK2 fused with a His tag at the N-terminus.
Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells. - 分子量
- 36.1 kDa
- UniProt
- P24941
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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