ATG3 Protein (AA 1-287) (His tag,T7 tag)
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- 抗原 See all ATG3 蛋白
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-287
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ATG3 protein is labelled with His tag,T7 tag.
- 原理
- Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)
- 序列
- GSMQNVINTV KGKALEVAEY LTPVLKESKF KETGVITPEE FVAAGDHLVH HCPTWQWATG EELKVKAYLP TGKQFLVTKN VPCYKRCKQM EYSDELEAII EEDDGDGGWV DTYHNTGITG ITEAVKEITL ENKDNIRLQD CSALCEEEED EDEGEAADME EYEESGLLET DEATLDTRKI VEACKAKTDA GGEDAILQTR TYDLYITYDK YYQTPRLWLF GYDEQRQPLT VEHMYEDISQ DHVKKTVTIE NHPHLPPPPM CSVHPCRHAE VMKKIIETVA EGGGELGVHM YLLIFLKFVQ AVIPTIEYDY TRHFTM
- 产品特性
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Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ATG3 蛋白
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ATG3 Autophagy Related 3 (ATG3) (AA 1-287) protein (His tag)
ATG3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (GST tag)ATG3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-100) protein (GST tag)ATG3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (His tag)ATG3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314), (full length) protein (His-SUMO Tag)ATG3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
ATG3 Autophagy Related 3 (ATG3) protein (Myc-DYKDDDDK Tag)ATG3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- 别名
- Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3 (ATG3 产品)
- 别名
- atg3 Protein, im:6910145 Protein, wu:fb15g12 Protein, zgc:64156 Protein, APG3 Protein, APG3-LIKE Protein, APG3L Protein, PC3-96 Protein, 2610016C12Rik Protein, Apg3l Protein, Atg3l Protein, PIG-1 Protein, Pig1 Protein, ATATG3 Protein, K11J9.1 Protein, K11J9_1 Protein, autophagy related 3 Protein, autophagy 3 (APG3) Protein, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Protein, atg3 Protein, ATG3 Protein, Atg3 Protein
- 背景
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Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-His287) of Human ATG3 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG3 (ATG3) is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. ATG3 as a E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. As an autocatalytic E2-like enzyme, ATG3 also can catalyzes the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy. - 分子量
- 36 kDa
- UniProt
- Q9NT62
- 途径
- Autophagy
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